Constitutive expression of various chemokine genes in human T-cell lines infected with human T-cell leukemia virus type 1: role of the viral transactivator Tax.

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

Constitutive expression of various chemokine genes in human T-cell lines infected with human T-cell leukemia virus type 1: role of the viral transactivator Tax.

Int J Cancer. 1996 Mar 28;66(1):124-9. Unique Identifier : AIDSLINE MED/96183757
Baba M; Imai T; Yoshida T; Yoshie O; Shionogi Institute for Medical Science, Osaka, Japan.

Abstract: We examined the genetic expression of 2 CXC chemokines (IL-8, IP-10), 5 CC chemokines (MCP-1, MIP-lalpha, MIP-1beta, RANTES, 1309) and 1 C chemokine (SCM-1/lymphotactin/ATAC) in various human T-cell lines. By Northern blot analysis, HTLV-1-positive T-cell lines were found to express a number of chemokine genes at variable levels and in different combinations. However, none of the chemokine genes was expressed in HTLV-1-negative T-cell lines. We further confirmed secretion of 3 chemokines (IL-8, MIP-1alpha and RANTES) by some HTLV-1-positive T-cell lines. To examine the role of the HTLV-1-encoded transactivator Tax in the induction of these chemokine genes, we used JPX-9 and JPX-M, which were stably transformed with tax and non-functional tax, respectively, under the control of a metallothionein promoter. Induction of tax in JPX-9 with Cd2+ was accompanied by rapid induction of IL-8, IP-10, MIP-1alpha, MIP-1beta, 1309 and SCM-1 as determined by reverse transcription PCR. No such induction was seen in JPX-M. We thus suggest that Tax is, at least in part, responsible for constitutive expression of certain chemokine genes in HTLV-1-infected T cells. Aberrant production of various chemokines by HTLV-1- infected T cells may impact on the pathophysiology of HTLV-1-associated diseases.

Keywords: Cells, Cultured Chemokines/*GENETICS Gene Expression Regulation, Neoplastic Gene Expression Regulation, Viral Gene Products, tax/*PHYSIOLOGY *Genes, pX Human HTLV-I/*GENETICS HTLV-I Infections/*MICROBIOLOGY RNA, Messenger/GENETICS T-Lymphocytes/*PHYSIOLOGY Tumor Cells, Cultured JOURNAL ARTICLE
960830
M9681206

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