Detection of human T-lymphotropic virus type-I DNA and mRNA in the lymph nodes; using polymerase chain reaction in situ hybridization (PCR/ISH) and reverse transcription (RT-PCR/ISH).

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

Detection of human T-lymphotropic virus type-I DNA and mRNA in the lymph nodes; using polymerase chain reaction in situ hybridization (PCR/ISH) and reverse transcription (RT-PCR/ISH).

Int J Cancer. 1996 Mar 28;66(1):18-23. Unique Identifier : AIDSLINE MED/96183740
Ohshima K; Suzumiya J; Izumo S; Mukai Y; Tashiro K; Kikuchi M; Department of Pathology, School of Medicine, Fukuoka University,; Japan.

Abstract: To examine the relationship between human T-lymphotrophic virus type I (HTLV-I) proviral DNA and its expression in the lymph nodes, HTLV-I DNA and tax/rex mRDA were directly amplified by polymerase chain reaction in situ hybridization (PCR/ISH), and reverse transcription (RT)-PCR/ISH [RT-PCR/ISH]. We studied 24 lymph nodes from patients with adult T-cell leukemia/lymphoma (ATLL), incipient ATLL (I-ATLL), and HTLV-I associated lymphadenitis dermatopathic type (HAL-D) and enlarged paracortical type (HAL-EP). In ATLL, 40-60% of the nucleated cells were positive for for HTLV-I proviral DNA by PCR/ISH, while in I-ATLL and HAL, respectively 5-20% and less than 1-5% of cells were positive. The number of mRNA expressing cells was smaller than that of the proviral DNA-positive cells. The mRNA-expressing cells varied in number among the ATLL and I-ATLL cases, while they were only rarely observed in HAL-D and HAL-EP. These results show that HTLV-I infection and activation might increase with malignant transformation of the target T helper cells.

Keywords: Adult Aged DNA, Viral/*ANALYSIS Female Gene Expression Regulation, Neoplastic Gene Expression Regulation, Viral Human HTLV-I/*GENETICS HTLV-I Infections/*DIAGNOSIS/GENETICS In Situ Hybridization/*METHODS Leukemia, T-Cell/*MICROBIOLOGY Lymph Nodes/*MICROBIOLOGY Male Middle Age Polymerase Chain Reaction/*METHODS RNA, Viral/*ANALYSIS JOURNAL ARTICLE
960830
M9681205

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