Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.
Development of CD8 alpha alpha+ intestinal intraepithelial T cells in beta 2-microglobulin- and/or TAP1-deficient mice.
J Immunol. 1996 Apr 15;156(8):2710-5. Unique Identifier : AIDSLINE MED/96183222 Fujiura Y; Kawaguchi M; Kondo Y; Obana S; Yamamoto H; Nanno M; Ishikawa H; Department of Microbiology, Keio University School of Medicine,; Tokyo, Japan.
Abstract:
The development of CD8+ intestinal intraepithelial T lymphocytes (IEL) was analyzed in mice that are deficient in the expression of MHC class I molecules, owing to either a mutated beta 2-microglobulin (beta 2m) gene or a mutated transporter associated with Ag processing 1 (TAP1) gene, and in mice doubly homozygous for beta 2m and TAPI mutations. In all mutant mice, the population size of major CD8 alpha alpha+ and CD8 alpha beta+ alpha beta-IEL subsets was reduced drastically, and this resulted in a conspicuous decrease in the total number of alpha beta-IEL. Concomitantly, a compensatory two- to threefold increase in the number of gamma delta-IEL consisting mostly of CD8 alpha alpha+ subset was noted. In radiation bone marrow chimeras, this wild-type/mutant phenotype was determined by the genotype of radioresistant host cells, but was not determined by the genotype of reconstituting bone marrow-derived cells. In beta 2m X TCR-delta double mutant mice, however, the CD8 alpha alpha+ but not CD8 alpha beta+ alpha beta-IEL subset expanded dramatically. Thus, in the absence of gamma delta-IEL, alpha beta-IEL in beta 2m-deficient mice outnumbered those in wild-type littermates. These results indicate that the generation of CD8 alpha alpha+ lymphocyte population of alpha beta- and gamma delta-IEL is not dependent, but that of CD8 alpha beta+ lymphocyte population of alpha beta-IEL is dependent on beta 2m- and/or TAP1-dependent MHC class I molecules, expressed by the controlling cells present in the anatomical site, where the development of IEL takes place.
Keywords: beta 2-Microglobulin/*DEFICIENCY/*GENETICS Animal Antigens, CD8/*GENETICS/RADIATION EFFECTS ABC Transporters/*GENETICS Base Sequence Bone Marrow/IMMUNOLOGY Cell Differentiation/IMMUNOLOGY CD8-Positive T-Lymphocytes/*CLASSIFICATION/IMMUNOLOGY/RADIATION EFFECTS Epithelium/CYTOLOGY/IMMUNOLOGY/RADIATION EFFECTS Female Histocompatibility Antigens Class I/GENETICS Immunophenotyping Intestinal Mucosa/*CYTOLOGY/IMMUNOLOGY/RADIATION EFFECTS Lymphocyte Transformation/GENETICS Male Mice Mice, Inbred C57BL Mice, Mutant Strains Molecular Sequence Data Radiation Chimera Receptors, Antigen, T-Cell, alpha-beta/GENETICS Receptors, Antigen, T-Cell, gamma-delta/GENETICS Support, Non-U.S. Gov't Thymus Gland/CYTOLOGY JOURNAL ARTICLE 960830
M9681184
AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.
Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.
