Interleukin (IL) 15/IL-T production by the adult T-cell leukemia cell line HuT-102 is associated with a human T-cell lymphotrophic virus type I region /IL-15 fusion message that lacks many upstream AUGs that normally attenuates IL-15 mRNA translation.

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2897-902. Unique Identifier : AIDSLINE MED/96181504
Bamford RN; Battiata AP; Burton JD; Sharma H; Waldmann TA; Metabolism Branch, National Cancer Institute, National Institutes; of Health, Bethesda, MD 20892-1374, USA.

Abstract: We reported previously that the human T-cell lymphotrophic virus type I (HTLV-I)-associated adult T-cell leukemia line HuT-102 produces a cytokine designated interleukin (IL) T that requires interleukin (IL) 2 receptor beta-subunit expression for its action. Using anti-cytokine antibodies, we demonstrated that IL-T is identical to the simultaneously described IL-15. When compared to activated monocytes, IL-15 mRNA expression was 6- to 10-fold greater in HuT-102 cells. The predominant IL-15 message from HuT-102 is a chimeric mRNA joining a segment of the R region of the long terminal repeat of HTLV-I and the 5'-untranslated region (UTR) of IL-15. Normally, by alternative splicing, this 118-nucleotide R element represents the most 5' region of several HTLV-I transcripts including tax, rex, and env. The introduction of the R element eliminated over 200 nucleotides of the IL-15 5'-UTR, including 8 of 10 upstream AUGs that are present in normal IL-15 messages. On analysis of the 5'-UTR of normal IL-15, we demonstrated that the presence of these 10 upstream AUGs interferes with IL-15 mRNA translation. Thus, IL-15 synthesis by the adult T-cell leukemia line HuT- 102 involves an increase in IL-15 mRNA transcription and translation secondary to the production of an HTLV-I R element fusion message that lacks many upstream AUGs.

Keywords: Adult Base Sequence Cell Line Chimeric Proteins/*BIOSYNTHESIS Codon *Gene Expression Regulation, Viral Human HTLV-I/GENETICS/*IMMUNOLOGY Interleukins/*BIOSYNTHESIS Leukemia-Lymphoma, T-Cell, Acute, HTLV-I-Associated/*IMMUNOLOGY Lipopolysaccharides/PHARMACOLOGY Lymphocyte Transformation Molecular Sequence Data Monocytes/IMMUNOLOGY RNA, Messenger/METABOLISM T-Lymphocytes/DRUG EFFECTS/*IMMUNOLOGY T-Lymphocytes, Cytotoxic/IMMUNOLOGY *Translation, Genetic JOURNAL ARTICLE
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M9681148

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