Amphibian transcription factor IIIA proteins contain a sequence element functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev.

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

Amphibian transcription factor IIIA proteins contain a sequence element functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev.

Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2936-40. Unique Identifier : AIDSLINE MED/96181511
Fridell RA; Fischer U; Luhrmann R; Meyer BE; Meinkoth JL; Malim MH; Cullen BR; Howard Hughes Medical Institute, Duke University Medical Center,; Durham, NC 27710, USA.

Abstract: The human immunodeficiency virus type 1 (HIV-1) Rev protein is required for nuclear export of late HIV-1 mRNAs. This function is dependent on the mutationally defined Rev activation domain, which also forms a potent nuclear export signal. Transcription factor IIIA (TFIIIA) binds to 5S rRNA transcripts and this interaction has been proposed to play a role in the efficient nuclear export of 5S rRNA in amphibian oocytes. Here it is reported that amphibian TFIIIA proteins contain a sequence element with homology to the Rev activation domain that effectively substitutes for this domain in inducing the nuclear export of late HIV-1 mRNAs. It is further demonstrated that this TFIIIA sequence element functions as a protein nuclear export signal in both human cells and frog oocytes. Thus, this shared protein motif may play an analogous role in mediating the nuclear export of both late HIV-1 RNAs and 5S rRNA transcripts.

Keywords: Amino Acid Sequence Animal Bufonidae Cell Nucleus/METABOLISM Comparative Study DNA-Binding Proteins/BIOSYNTHESIS/*CHEMISTRY/*METABOLISM Female Gene Expression Regulation Gene Products, rev/*CHEMISTRY/*METABOLISM Human HIV-1/*METABOLISM HIV-2/*METABOLISM Molecular Sequence Data Oocytes/PHYSIOLOGY Recombinant Fusion Proteins/BIOSYNTHESIS/CHEMISTRY/METABOLISM RNA, Messenger/METABOLISM RNA, Ribosomal, 5S/BIOSYNTHESIS Sequence Homology, Amino Acid Species Specificity Transcription Factors/BIOSYNTHESIS/*CHEMISTRY/*METABOLISM Transcription, Genetic Xenopus laevis JOURNAL ARTICLE
960830
M9681147

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