Protein stability influences human immunodeficiency virus type 2 Vpr virion incorporation and cell cycle effect. NLM AIDSLINE Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

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Protein stability influences human immunodeficiency virus type 2 Vpr virion incorporation and cell cycle effect.

Virology. 1996 Apr 15;218(2):326-34. Unique Identifier : AIDSLINE MED/96193744
Kewalramani VN; Park CS; Gallombardo PA; Emerman M; Fred Hutchinson Cancer Research Center, Division of Molecular; Medicine, Seattle, Washington 98104, USA.

Abstract: Using recombinant proteins as standards, we calculated the amount of Vpr and Vpx present in HIV-2ROD particles. We find 2000-3000 copies of Vpx per particle but only 40-50 copies of Vpr. We investigated the reasons for this discrepancy between Vpx and Vpr and found that viral factors, including HIV-2 Vpx, do not restrict its incorporation. Instead, the accumulation of HIV-2ROD Vpr during infection is restricted by a short protein half-life which acts to limit its virion incorporation. The half-life of HIV-2 Vpr was calculated to be about 90 min, while HIV-2 Vpx and HIV-1 Vpr had half-lifes of 36 and 20 hr, respectively. Moreover, while both HIV-1 Vpr and HIV-2 Vpr cause cells to accumulate in G2 of the cell cycle, the effect of HIV-2 Vpr is attenuated relative to HIV-1 Vpr. Thus, protein stability correlates with both the function of Vpr and its virion incorporation.
Keywords: Cell Cycle/PHYSIOLOGY Cell Line Gene Products, vpr/*ANALYSIS/METABOLISM/PHYSIOLOGY Half-Life Hela Cells Human HIV-1/METABOLISM HIV-2/*CHEMISTRY/METABOLISM Recombinant Fusion Proteins Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. T-Lymphocytes Viral Regulatory Proteins/ANALYSIS/METABOLISM/PHYSIOLOGY Virion/*CHEMISTRY JOURNAL ARTICLEKWDcellcycle/physiologycelllinegeneproducts,vpr/KWDanalysis/metabolism/physiologyhalf-lifehelacellshumanhiv-1/metabolismhiv-2/KWDchemistry/metabolismrecombinantfusionproteinssupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDt-lymphocytesviralregulatoryproteins/analysis/metabolism/physiologyvirion/KWDchemistryjournalarticle
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Copyright © 1996 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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