Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.
The amount of host HLA-DR proteins acquired by HIV-1 is virus strain- and cell type-specific.
Virology. 1996 Apr 15;218(2):372-81. Unique Identifier : AIDSLINE MED/96193749 Cantin R; Fortin JF; Tremblay M; Centre de Recherche en Infectiologie, Centre Hospitalier de; l'Universite Laval, Ste-Foy, Quebec, Canada.
Abstract:
We semiquantitatively monitored the incorporation of host membrane proteins on different strains of human immunodeficiency virus type 1 (HIV-1) grown in several human CD4+ lymphoid cell lines and in primary mitogen-stimulated peripheral blood mononuclear cells. The relative amounts of virally acquired cell proteins were estimated by the ability of HIV-1 to be captured by magnetic beads coated with monoclonal antibodies. Here we report that, among host surface proteins studied, HLA-DR molecules were the most abundant virion-bound host molecules. We have also found that, in contrast to previous studies, HLA-DP and -DQ isotypes were also present on virus progeny. More importantly, we determined that the relative levels of virally acquired host HLA-DR proteins, as estimated by capture with immunomagnetic beads, greatly differed depending on the virus strain and the producer cell. These observations extend beyond already published results and suggest that the process of incorporation of cellular molecules on newly released virus particles is a phenomenon that relies on both the virus strain and producer cell line. These in vitro observations are of prime importance considering that virus-acquired host molecules have been recently shown to affect the biology of HIV.
Keywords: Antibodies, Monoclonal Antigens, CD4/ANALYSIS Cell Line Cells, Cultured CD4-Positive T-Lymphocytes/*VIROLOGY Histocompatibility Antigens Class I/ANALYSIS Human HIV Core Protein p24/ANALYSIS HIV-1/*PHYSIOLOGY HLA-DP Antigens/ANALYSIS HLA-DQ Antigens/ANALYSIS HLA-DR Antigens/*ANALYSIS Immunomagnetic Separation Intercellular Adhesion Molecule-1/ANALYSIS Leukocytes, Mononuclear/VIROLOGY Species Specificity Support, Non-U.S. Gov't Virion/*CHEMISTRY Virus Assembly JOURNAL ARTICLE 960830
M9681130
AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.
Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.
