Human immunodeficiency virus type 1 integrase stabilizes a linearized HIV-1 LTR plasmid in vivo. NLM AIDSLINE Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

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Human immunodeficiency virus type 1 integrase stabilizes a linearized HIV-1 LTR plasmid in vivo.

Biochem Mol Biol Int. 1995 Jul;36(4):745-58. Unique Identifier : AIDSLINE MED/96057063
Faust EA; Acel A; Udashkin B; Wainberg MA; Lady Davis Institute for Medical Research, SMBD-Jewish General; Hospital, Montreal, Quebec, Canada.

Abstract: A mammalian expression vector designed for production of HIV-1 integrase was found to enhance the stability of a linear reporter plasmid in COS-7 cells. The effect is strictly dependent on coexpression of the HIV-1 rev gene and on the inclusion of U3 and U5 portions of the HIV-1 LTR in the reporter plasmid. Integrase point mutations P109S and D116N drastically reduced stabilization whereas T115A and D64A had little or no effect. Immunoblot analysis revealed the presence of a 32-34kDa integrase protein in extracts of transfected COS-7 cells and of wild type and mutant integrase proteins at comparable levels. We conclude that integrase acts in trans in COS-7 cells, possibly by binding to the HIV-1 LTR in the plasmid. This transfection system may be useful for studying factors that stabilize the HIV-1 DNA genome prior to its integration into the host cell chromosome.
Keywords: Amino Acid Sequence Animal Base Sequence Cell Line Cercopithecus aethiops DNA Nucleotidyltransferases/ANALYSIS/BIOSYNTHESIS/*METABOLISM *Genes, rev Human *HIV Long Terminal Repeat HIV-1/*ENZYMOLOGY/*GENETICS Molecular Sequence Data Molecular Weight Mutagenesis, Insertional Mutagenesis, Site-Directed Oligodeoxyribonucleotides Plasmids/*METABOLISM Point Mutation Recombinant Proteins/ANALYSIS/BIOSYNTHESIS/METABOLISM Restriction Mapping Support, Non-U.S. Gov't Transfection Virus Integration JOURNAL ARTICLEKWDaminoacidsequenceanimalbasesequencecelllinecercopithecusaethiopsdnanucleotidyltransferases/analysis/biosynthesis/KWDmetabolismKWDgenes,revhumanKWDhivlongterminalrepeathiv-1/KWDenzymology/KWDgeneticsmolecularsequencedatamolecularweightmutagenesis,insertionalmutagenesis,site-directedoligodeoxyribonucleotidesplasmids/KWDmetabolismpointmutationrecombinantproteins/analysis/biosynthesis/metabolismrestrictionmappingsupport,non-uKWDsKWDgov'ttransfectionvirusintegrationjournalarticle
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