SIV infection of monkey spleen cells including follicular dendritic cells in different stages of disease. NLM AIDSLINE Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

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SIV infection of monkey spleen cells including follicular dendritic cells in different stages of disease.

J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Jan 1;11(1):1-9. Unique Identifier : AIDSLINE MED/96130041
Stahmer I; Ordonez C; Popovic M; Mesquita R; Ekman M; Albert J; Putkonen P; Bottiger D; Biberfeld G; Biberfeld P; Immunopathology Laboratory, Karolinska Institute, Stockholm,; Sweden.

Abstract: Immunoaffinity enriched spleen follicular dendritic cells (FDCs), lymphocytes, and macrophages from SIVsm-inoculated cynomolgus monkeys (Macaca fascicularis) at different stages of disease were compared for latent and productive SIV infection. Analysis of FDCs by in situ hybridization, electron microscopy, and coculture assays indicated that comparatively high levels of virus were associated with the FDC fraction. Polymerase chain reaction (PCR) and RT-PCR results revealed that the levels for SIVpol DNA did not correlate with the level of env mRNA in the various cell subsets, suggesting differences in latency. Limiting dilution assays for spliced env mRNA showed a 10-100-fold higher amount of env mRNA in FDCs than in other spleen cell subsets early during SIV infection. At late stages of disease, the number of productively infected FDCs significantly decreased in parallel with a marked reduction of the FDC network and follicular involution. Our findings indicate that destruction of FDCs probably reflects a cytopathic effect of SIV and/or the activity of specific antiviral cytotoxic T lymphocytes.
Keywords: Animal Base Sequence CD4 Lymphocyte Count Dendritic Cells/ULTRASTRUCTURE/*VIROLOGY DNA Primers/CHEMISTRY DNA Replication DNA, Viral/ANALYSIS Genes, env/GENETICS Genes, pol/GENETICS In Situ Hybridization Lymphocytes/ULTRASTRUCTURE/VIROLOGY Macaca fascicularis Macrophages/ULTRASTRUCTURE/VIROLOGY Molecular Sequence Data Polymerase Chain Reaction RNA, Messenger/ANALYSIS Simian Acquired Immunodeficiency Syndrome/*PHYSIOPATHOLOGY Spleen/CYTOLOGY/ULTRASTRUCTURE/*VIROLOGY Support, Non-U.S. Gov't SIV/GENETICS/*PHYSIOLOGY/ULTRASTRUCTURE Virus Replication JOURNAL ARTICLEKWDanimalbasesequencecd4lymphocytecountdendriticcells/ultrastructure/
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Copyright © 1996 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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