Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.
Characterization of an antigen shared by human thymic epithelium and human T cell leukemia virus p19 Gag protein.
J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Jan 1;11(1):10-9. Unique Identifier : AIDSLINE MED/96130042 Palker TJ; Singer KH; Vahlne A; Department of Medicine, Duke University Medical Center, Durham,; North Carolina, USA.
Abstract:
The molecular basis for cross-reactive antibody binding to human T cell leukemia virus type I (HTLV-I) p19 core protein and human thymic epithelium has been defined with two monoclonal antibodies (mAbs), 12/1-2 and 13B12, raised to HTLV-I p19. The mAb 12/1-2 has previously been shown to react with HTLV-I p19, HTLV-II p22, and antigens of normal human thymic epithelium, placenta, and foreskin, whereas mAb 13B12 binds only to the carboxyl terminus of HTLV-I p19. In the present study, mAb 12/1-2 bound to a subset of Triton X-100-insoluble intermediate filaments in human thymic epithelium also recognized by antikeratin antibodies AE1 and AE3. The mAb 12/1-2 also reacted in Western blot assays with proteins of 54, 46, and 40 kDa present in extracts of human thymic epithelium and with hexameric peptides containing overlapping sequences of HTLV-I p19 with the amino acids IPP (amino acids 117-119). In contrast, the HTLV-I-specific mAb 13B12 did not bind to human thymic epithelium and reacted with a single hexameric peptide containing the carboxy-terminal HTLV-I p19 sequence IPPPYV (amino acids 117-122). Binding of mAb 12/1-2 to thymic epithelium could be inhibited by adsorption with peptide SP-79 containing a C-terminal sequence (amino acids 112-125) of p19. The crossreactive IPP site is within a region of p19 that has been previously shown to be highly immunogenic in HTLV-I-infected individuals and that is also encoded by genes or mRNA of human cytokeratin 17, keratin 4, epidermal cytokeratin 2, and 50-kDa type I epidermal keratin. Thus, our studies define the sequence of a cross-reactive antigen on HTLV-I p19 that is also associated with keratin intermediate filaments from human thymic epithelium and other normal human tissues and that could serve as a focus of an autoimmune response during HTLV-I infection.
Keywords: Amino Acid Sequence Animal Antibodies, Monoclonal Blotting, Western Cells, Cultured Child Cloning, Molecular Cross Reactions/IMMUNOLOGY Epithelium/IMMUNOLOGY Epitopes/CHEMISTRY/IMMUNOLOGY Fluorescent Antibody Technique Gene Products, gag/CHEMISTRY/*IMMUNOLOGY Human Hybridomas HTLV-I/*IMMUNOLOGY HTLV-I Antigens/CHEMISTRY/*IMMUNOLOGY Keratin/CHEMISTRY/IMMUNOLOGY Male Mice Molecular Sequence Data Rabbits Radioimmunoassay Retroviridae Proteins, Oncogenic/CHEMISTRY/*IMMUNOLOGY Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Thymus Gland/*IMMUNOLOGY JOURNAL ARTICLE
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Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.
