Effect of CD8 T cell lines derived from vertically HIV infected children on HIV replication in naturally infected cells. American Pediatric Society 104th annual meeting and Society for Pediatric Research 63rd annual meeting; 1994 May 2-5; Seattle.

Important note: Information in this article was accurate in 1995. The state of the art may have changed since the publication date.

Pediatr AIDS HIV Infect. 1994 Oct;5(5):314 (unnumbered abstract). Unique Identifier : AIDSLINE AIDS/95330396
Heerema AE; Holmes DR; Sullivan JL; Luzuriaga K; Dept. of Pediatrics, Univ. of Mass. Medical Center, Worcester,; USA.

Abstract: The CD8 T cell population undergoes rapid early expansion in vertical HIV infection, yet the functional capabilities of those CD8 T cells are not well defined. Our lab has previously described a deficient CD8 T cell mediated HIV-specific CTL response in vertically-infected children. Several groups have reported a specific anti-viral effect of CD8 T cells from HIV-infected adults. CD8 lymphocytes from these persons have been shown to inhibit viral replication in infected cell lines and PBMC. This phenomenon appears to be mediated by release of a soluble anti-viral factor by the CD8 T cells. We have begun to investigate the ability of CD8 T cells from vertically infected children to inhibit viral replication in autologous and heterologous naturally HIV-infected CD4 T cell lines. Cell lines were derived from vertically infected children ranging from 3 mos. to 7 yrs. of age, and on HIV-uninfected controls through stimulation of PBMC with PHA and IL-2, and subsequent selection for CD4 and CD8 fractions. HIV specific cytolytic potential of the CD8 T cell lines was assessed by combining them with autologous B-lymphoblastoid cell lines infected with vaccinia constructs expressing HIV-1 gene products. Cocultures were performed in triplicate in 96-well plates by combining 10(5) CD4 T cells per well with 0.1 x 10(5), 2 x 10(5), or 5 x 10(5) CD8 T cells. Inhibition of viral replication was assessed by supernatant p24 Ag, in situ hybridization, and PCR. Viral replication was inhibited in a dose dependent manner by CD8 T cell lines derived from five children; degree of inhibition varied among lines. No significant HLA restriction was observed for this anti-viral effect, nor did cells from HIV-infected children differ in anti-viral capability from those of uninfected controls. CD8 T cell lines from one child appeared to augment viral replication despite high cytolytic capabilities in the CTL assay. Further studies to elucidate the mechanism of CD8 T cell inhibition of viral replication are underway.

Keywords: Cell Line Child Child, Preschool CD4-Positive T-Lymphocytes/PHYSIOLOGY CD8-Positive T-Lymphocytes/*PHYSIOLOGY *Disease Transmission, Vertical Human HIV/*PHYSIOLOGY HIV Core Protein p24/ANALYSIS HIV Infections/*IMMUNOLOGY/TRANSMISSION/VIROLOGY In Situ Hybridization Infant Polymerase Chain Reaction *Virus Replication ABSTRACT JOURNAL ARTICLE
951030
M95A0912

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