Human T cells transformed in vitro by human T-cell leukemia virus (Meeting abstract).

Important note: Information in this article was accurate in 1995. The state of the art may have changed since the publication date.

Human T cells transformed in vitro by human T-cell leukemia virus (Meeting abstract).

2nd International Conference on Gene Regulation/Oncogenesis/AIDS, September 23-30, 1993, Loutraki, Greece, p. 34, 1993.. Unique Identifier : AIDSLINE ICDB/95606697
Taresis S; Parks W; Parks E; Persaud D; Munoz J; New York University School of Medicine, New York, NY

Abstract: To study early events associated with HTLV-1/2 infection and transformation it is necessary to isolate clonal populations of HTLV-exposed lymphocytes early after infection. Peripheral blood mononuclear cells (PBMNCs) were prestimulated prior to coculture with irradiated HTLV-1 positive C91/PL cells. PBMNCs (10(4)/well) were noted, respectively, as determined by HTLV-1 p24 production. All HTLV-positive cells grew continuously in the presence of IL-2 and were considered 'transformed'; no spontaneous growth of uninfected lymphocytes was found. Of 44 lymphocyte cultures phenotyped, all are CD3+ and B1-, 48% are CD4+, 11% are CD8+, and the remainder are mixed CD4+/CD8+ populations. Southern blot analysis for T cell receptor beta chain gene rearrangement of 14 cells lines that are clonal by cell surface analysis confirmed that 13 lines were indeed clonal. Digestion with EcoRI which does not cut within the HTLV coding sequence and analysis with a full-length viral probe showed that only two cultures had single integration sites and the remainder were oligoclonal at the earliest time point examined. Digestion with the enzyme SstI that cuts once in each long terminal repeat (LTR), revealed a defective provirus of 6.2 kb plus the full-length 8.5-kb provirus. While most of the cell lines contained both the full-length and the defective provirus, two cell lines contained only the full-length form and one of the monoclonal integrants contained only the defective provirus. Thus, HTLV-1 and HTLV-2 both transform comparably and are rarely clonal integrants.

Keywords: Blotting, Southern Cell Line, Transformed *Cell Transformation, Viral Human *HTLV-I *HTLV-II T-Lymphocytes/*PATHOLOGY ABSTRACT
950530
M9551043

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