IL-12 expression in AIDS-related Burkitt's lymphoma cell lines (AABCL) (Meeting abstract).

Important note: Information in this article was accurate in 1995. The state of the art may have changed since the publication date.

IL-12 expression in AIDS-related Burkitt's lymphoma cell lines (AABCL) (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 35:A2913 1994. Unique Identifier : AIDSLINE ICDB/95604598
Benjamin D; Sharma V; Kubin M; Baird M; Trinchieri G; Division of Hematology/Oncology, James Cancer Hospital and; Research Inst., The Ohio State Univ., Columbus, OH 43210

Abstract: IL-12 is a 70 kD heterodimer cytokine formed by 40 kD heavy chain (p40) and 35 kD light chain (p35). IL-12 was detected in EBV-transformed normal lymphoblastoid B cell lines (CLS) but not in Burkitt's lymphoma. Using PCR analysis we studied IL-12 expression in 21 B CLS obtained from patients with American Burkitt's, African Burkitt's and AIDS-related Burkitt's lymphoma in comparison with 6 EBV transformed B CLS. Whereas p35 mRNA expression was found in 27/27 CLS, p40 mRNA was detected only in the AABCL (6/6) and the normal lymphoblastoid B CLS (6/6). p40 mRNA was not detected in any of the 15 non-AIDS Burkitt's lymphoma CLS. 6/6 AABCL which were derived from two patients with AIDS and Burkitt's lymphoma, constitutively secreted large amounts of p40 (range: 428-17,331 pg/ml) and 2/6 AABCL secreted p70 (22-493 pg/ml), whereas the normal lymphoblastoid CLS secreted smaller amounts of p40 (range: less than 50 to 189 pg/ml) and secreted less than 20 pg/ml p70. Stimulation of the CLS with PMA induced 2- to 7-fold increase in p40 secretion and triggered the secretion of detectable p70 in additional 2 AABCL and in 4/6 normal lymphoblastoid CLS. These results, combined with our previous observation that the AABCL secrete large amounts of IL-10, which is known to inhibit IL-12, suggest that: (I) IL-10 does not negatively regulate IL-12 secreted by B cells derived from patients with AIDS and Burkitt's lymphoma. (II) The AABCL express unique pattern of B cell cytokines. (III) IL-12 p40 can be used as a marker to differentiate AABCL from non-AABCL.

Keywords: B-Lymphocytes/METABOLISM Burkitt's Lymphoma/*GENETICS Human Interleukin-10/GENETICS/METABOLISM Interleukin-12/*GENETICS Lymphoma, AIDS-Related/*GENETICS RNA, Messenger/METABOLISM ABSTRACT
950330
M9530837

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1995. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1995. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .