Inhibitory effects of caffeic acid phenethyl ester (CAPE) on tumor promotion in mouse epidermis and the synthesis of DNA and RNA in HeLa cells (Meeting abstract).

Important note: Information in this article was accurate in 1995. The state of the art may have changed since the publication date.

Inhibitory effects of caffeic acid phenethyl ester (CAPE) on tumor promotion in mouse epidermis and the synthesis of DNA and RNA in HeLa cells (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 35:A3725 1994. Unique Identifier : AIDSLINE ICDB/95605410
Huang MT; Ma W; Yen P; Xie JQ; Frenkel K; Grunberger D; Conney AH; Lab. for Cancer Res., Rutgers Univ., Piscataway, NJ 08855

Abstract: CAPE, which is found in propolis (a product of honeybee hives), selectively inhibits the growth of several transformed cell lines. Previous studies showed that CAPE inhibits 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation (ear edema) and increases in epidermal ornithine decarboxylase activity (Cancer Res 53:1255-61, 1993). We now report that CAPE also inhibits TPA-induced tumor promotion in mouse epidermis and the synthesis of DNA and RNA in HeLa cells. Topical application of 0.1 or 3 umol of CAPE with 5 nmol TPA to the backs of DMBA-initiated female CD-1 mice twice weekly for 20 weeks inhibited TPA-induced papillomas per mouse by 45% or 70%, respectively, and the papilloma volume per mouse was reduced by 53 and 74%, respectively. CAPE at 5, 10 or 20 ug/ml inhibited the incorporation of [3H]thymidine into the DNA of HeLa cells by 32, 75 or 96%, respectively, and incorporation of [3H]uridine into RNA was inhibited by 6, 18 or 54%, respectively. Incorporation of [3H]leucine into protein was not inhibited. The data indicate that CAPE is a potent inhibitor of TPA-induced tumor promotion in mouse epidermis and DNA synthesis in cultured HeLa cells.

Keywords: Animal Caffeic Acids/*PHARMACOLOGY Cell Transformation, Neoplastic/*DRUG EFFECTS Dose-Response Relationship, Drug DNA, Neoplasm/*ANTAGONISTS & INHIB Hela Cells Human Mice RNA, Neoplasm/*ANTAGONISTS & INHIB Skin Neoplasms/CHEMICALLY INDUCED/*PREVENTION & CONTROL Tetradecanoylphorbol Acetate Tumor Cells, Cultured/*DRUG EFFECTS ABSTRACT
950330
M9530827

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