Interactions among interferon-inducible genes (Meeting abstract).

Important note: Information in this article was accurate in 1995. The state of the art may have changed since the publication date.

Interactions among interferon-inducible genes (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 35:A3572 1994. Unique Identifier : AIDSLINE ICDB/95605257
Cha Y; Huston L; Deisseroth AB; UT M.D. Anderson Cancer Center, Houston, TX 77030

Abstract: The interferon genes are composed of a family of proteins involved in the regulation of cellular proliferation, immune response, response to viruses, and cell-cell interactions. Interferon-inducible genes (IIG), 9-27, 6-16, IRF-1, IRF-2, ISGF3, and ICSBP, have been cloned by several independent investigators. IRF-1 and IRF-2 are the transcriptional regulatory proteins which activate or limit respectively the duration of the activation of interferon gene expression by viruses, and have been proposed to perform a similar function for the interferon-inducible genes following exposure of cells to interferon. The interferon-inducible protein, 9-27, has been shown to inhibit Rev-mediated expression of genes important in the replication of the human immunodeficiency virus. ISGF3 is an immediate early response gene in the interferon signal transduction pathway. The actual function of 6-16 is unknown. All these IIGs have ability to bind to the interferon stimulatory response element (ISRE). In order to study the interaction and regulation of IIGs, we linked the promoter of human 9-27, 6-16, IRF-1, and IRF-2 to a luciferase reporter gene and subcloned IRF-1, ISGF3, and ICSBP cDNA into an eukaryotic expression vector. In a transient cotransfection assay, we analyzed the expression of the IIG promoters in the presence of overexpressed IIG proteins. We found that IRF-1 induced the expressions of IRF-2, 6-16, and 9-27 promoters. ISGF3 has no effect on the expression of IRF-1, IRF-2, 6-16, and 9-27 promoters. The data suggest that IRF-1, IRF-2, 6-16, and 9-27 may interact together through a complex mechanism in the regulation of these interferon-inducible gene expressions.

Keywords: DNA-Binding Proteins/*GENETICS Gene Expression Gene Products, rev/GENETICS HIV/GENETICS Interferons/*PHYSIOLOGY Phosphoproteins/*GENETICS Promoter Regions (Genetics) Signal Transduction Transcription Factors/*GENETICS ABSTRACT
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M9530823

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