Expression of the IL-2 receptor gamma chain on various populations in human peripheral blood. NLM AIDSLINE Important note: Information in this article was accurate in 1995. The state of the art may have changed since the publication date.

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Expression of the IL-2 receptor gamma chain on various populations in human peripheral blood.

Int Immunol. 1994 Aug;6(8):1273-7. Unique Identifier : AIDSLINE MED/95071959
Ishii N; Takeshita T; Kimura Y; Tada K; Kondo M; Nakamura M; Sugamura K; Department of Microbiology, Tohoku University School of Medicine,; Sendai, Japan.

Abstract: We have established two rat mAbs, TUGh4 and TUGh5, specific for the human gamma chain of the IL-2 receptor (IL-2R), which is known to be shared among receptors for IL-2, IL-4 and IL-7. The antibodies bound to cell lines transfected with the human gamma chain gene but not to their parental cell lines, and precipitated 65-70 and 80-90 kDa cell surface molecules from lysates of human T cells surface-labeled with Na125I and chemically cross-linked with [125I]IL-2 respectively. Flow cytometry with TUGh4 and TUGh5 detected the gamma chain in a wide variety of peripheral blood cell populations including CD4+ T cells, CD8+ T cells, CD20+ B cells, CD56+ natural killer cells, CD14+ monocytes and granulocytes, contrasting with expression of the alpha and beta chains of IL-2R.
Keywords: Antibodies, Monoclonal/IMMUNOLOGY Antigens, CD/IMMUNOLOGY CD4-Positive T-Lymphocytes/IMMUNOLOGY CD8-Positive T-Lymphocytes/IMMUNOLOGY Flow Cytometry Human Killer Cells, Natural/IMMUNOLOGY Leukocytes, Mononuclear/*IMMUNOLOGY Precipitin Tests Receptors, Interleukin-2/*BIOSYNTHESIS/IMMUNOLOGY Support, Non-U.S. Gov't JOURNAL ARTICLEKWDantibodies,monoclonal/immunologyantigens,cd/immunologycd4-positivet-lymphocytes/immunologycd8-positivet-lymphocytes/immunologyflowcytometryhumankillercells,natural/immunologyleukocytes,mononuclear/KWDimmunologyprecipitintestsreceptors,interleukin-2/KWDbiosynthesis/immunologysupport,non-uKWDsKWDgov'tjournalarticle
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M9530807

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