Important note: Information in this article was accurate in 1995. The state of the art may have changed since the publication date.
DNA inoculation induces protective in vivo immune responses against cellular challenge with HIV-1 antigen-expressing cells.
AIDS Res Hum Retroviruses. 1994;10 Suppl 2:S35-41. Unique Identifier : AIDSLINE MED/95169512 Wang B; Merva M; Dang K; Ugen KE; Boyer J; Williams WV; Weiner DB; Department of Medicine, University of Pennsylvania School of; Medicine, Philadelphia 19104.
Abstract:
Direct DNA inoculation induces immune responses through the delivery of nonreplicating transcription units that drive the synthesis of specific foreign proteins within the inoculated host. These proteins are processed within host cells and through association with relevant MHC antigens that can become the subject of immune surveillance and elicit immune responses against pathogens. Direct introduction of DNA into mice has been reported to be antigenic as demonstrated by the use of this technique to develop immune responses against human growth hormone, influenza proteins, as well as HIV-1 proteins. Most recently the demonstration of the use of this technology to produce anti-HIV-1 immune responses has been reported in nonhuman primates. Accordingly a more detailed analysis of this technology could generate important insight into the generality of this approach for immune therapy or vaccine design. In this article we further our investigation of direct DNA inoculation as a tool for induction of relevant immune responses against HIV-1 in vivo. We demonstrate expression of HIV-1 antigens in the inoculated muscle of animals. Inoculated animals demonstrate significant cytotoxic T cell responses against HIV-1 antigen-expressing targets. Furthermore, using a novel challenge system, we demonstrate that the majority of immunized animals can reject lethal, HIV-1 antigen-expressing cell challenge in an antigen-specific manner. This technology has relevance for the development of immunization strategies against HIV as it provides for specific antigen production in vivo without the use of infectious agents.
Keywords: Animal Antigen-Presenting Cells/*IMMUNOLOGY AIDS Vaccines/*PHARMACOLOGY DNA, Viral/ADMINISTRATION & DOSAGE/GENETICS/*IMMUNOLOGY Gene Products, env/BIOSYNTHESIS/GENETICS/IMMUNOLOGY Genetic Vectors HIV-1/GENETICS/*IMMUNOLOGY Immunity, Cellular Immunization Mice Mice, Inbred BALB C Muscles/IMMUNOLOGY/VIROLOGY Neoplasms, Experimental/IMMUNOLOGY/VIROLOGY Protein Precursors/GENETICS/IMMUNOLOGY Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. T-Lymphocytes, Cytotoxic/IMMUNOLOGY JOURNAL ARTICLE 950630
M9561104
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Important note: Information in this article was accurate in 1995. The state of the art may have changed since the publication date.
