[In vitro synergic lymphocyte stimulation in HIV-1 infected patients using inosine derivatives and radio-detoxified endotoxin]

Important note: Information in this article was accurate in 1995. The state of the art may have changed since the publication date.

[In vitro synergic lymphocyte stimulation in HIV-1 infected patients using inosine derivatives and radio-detoxified endotoxin]

Orv Hetil. 1995 Mar 19;136(12):653-9. Unique Identifier : AIDSLINE MED/95223665
Ongradi J; Hadden JW; Bertok L; Specter S; Nelson R; Friedman H; Mikrobiologiai Intezet, Semmelweis Orvostudomanyi Egyetem,; Budapest.

Abstract: Restoration of immune functions through promoting cell cycle might delay acquired immunodeficiency syndrome development. Therefore, stimulation of peripheral lymphocytes of human immunodeficiency virus-1 infected patients in successive clinical stages was studied by phytohaemagglutinin and other stimulants. In vitro blastogenesis was quantitated by 3H-thymidine uptake. Stimulation by phytohaemagglutinin decreased in patients with AIDS related complex to 63.1%, with AIDS to 13.6% of control values. Small amount of recombinant interleukin-2 or indomethacin solely not promoting lymphocytes, increased response to phytohaemagglutinin minimally. Alone ineffective methyl-ester and methyl-phosphonate inosine derivatives augmented phytohaemagglutinin-response of controls and patients with AIDS related complex by approx. 1.5-fold, but the effect in the case of AIDS patients was minimal. Radio-detoxified endotoxin alone or in combination with phytohaemagglutinin stimulated lymphocytes of both controls and patients with AIDS related complex slightly. Lymphocyte stimulation of patients with AIDS related complex was augmented in concentration-dependent manner, and by synergic effect it approached phytohaemagglutinin-stimulated blastogenesis of controls. Anergy due to human immunodeficiency virus-1 infection damages synchronisation of secondary messenger systems induced on cell surface receptors, therefore their selective influence by recombinant interleukin-2 or indomethacin is less efficient. Inosine derivatives promote cell cycle by inhibiting cyclic adenosine 3',5'-monophosphate production. In the early stage of virus infection, radio-detoxified endotoxin might bind to receptors of immature T cells and facilitate cell cycle through cyclic guanosine 3',5'-monophosphate stimulation. The clinical trials of radio-detoxified endotoxin (Tolerin) have already been launched.

Keywords: Acquired Immunodeficiency Syndrome/*THERAPY Adult AIDS-Related Complex/*THERAPY Endotoxins/RADIATION EFFECTS/*THERAPEUTIC USE English Abstract Human HIV Infections/*THERAPY HIV-1 Immunization Inosine/ADMINISTRATION & DOSAGE Interleukin-2/ADMINISTRATION & DOSAGE Lymphocytes/*DRUG EFFECTS Male Phytohemagglutinins/ADMINISTRATION & DOSAGE Thymidine/ADMINISTRATION & DOSAGE JOURNAL ARTICLE REVIEW REVIEW LITERATURE
950730
M9570571

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