Phenotype and suppressor activity of T-lymphocyte clones extracted from lesions of oral lichen planus.

Important note: Information in this article was accurate in 1995. The state of the art may have changed since the publication date.

Phenotype and suppressor activity of T-lymphocyte clones extracted from lesions of oral lichen planus.

Br J Dermatol. 1994 Sep;131(3):319-24. Unique Identifier : AIDSLINE MED/95001448
Sugerman PB; Savage NW; Seymour GJ; Department of Dentistry, University of Queensland, St Lucia,; Australia.

Abstract: Lymphocytes were extracted from six biopsy specimens of oral lichen planus. T-lymphocyte lines were expanded in culture with phytohaemagglutinin and interleukin 2, and cloned by limiting dilution. Fifteen T-cell clones were isolated with a probability of clonality of 96.3%. The majority of clones (n = 13) expressed the alpha beta T-cell receptor, and of these, 11 were CD8+ and two were CD4+. Two clones were CD4- and CD8-, and expressed the gamma delta T-cell receptor. The ability of these clones (effectors) to suppress concanavalin-A-stimulated proliferation of autologous lesional T-cell lines (responders) was assessed. Maximum suppressor activity ranged from 17 to 100%. The majority of clones (n = 12), including a CD3+ CD4+ CD8-alpha beta+ clone, displayed suppressor activity which was proportional to the effector to responder ratio. A CD3+CD4+CD8-alpha beta+ clone and a CD3+CD4-CD8-gamma delta+ clone displayed substantial helper activity at higher effector to responder ratios. These results demonstrate differential helper and suppressor activity of T-lymphocyte clones extracted from oral lichen planus lesions. The balance between help and suppression may be a fundamental determinant of immunological activity within the lymphocytic infiltrate of oral lichen planus, and hence may dictate the clinical behaviour of the disease.

Keywords: Adult Aged CD4-Positive T-Lymphocytes/CHEMISTRY/*IMMUNOLOGY CD8-Positive T-Lymphocytes/CHEMISTRY/*IMMUNOLOGY Female Human Lichen Planus, Oral/*IMMUNOLOGY Lymphocyte Transformation/IMMUNOLOGY Middle Age Phenotype Receptors, Antigen, T-Cell, alpha-beta/ANALYSIS Receptors, Antigen, T-Cell, gamma-delta/ANALYSIS Support, Non-U.S. Gov't T-Lymphocytes, Helper-Inducer/IMMUNOLOGY JOURNAL ARTICLE
950130
M9510816

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