Gene therapeutics: methods and applications of direct gene transfer.

Important note: Information in this article was accurate in 1995. The state of the art may have changed since the publication date.

Gene therapeutics: methods and applications of direct gene transfer.

Gene Therapeutics: Methods and Applications of Direct Gene Transfer. Wolff JA, eds. Boston, Birkhauser, 417 p., 1994.. Unique Identifier : AIDSLINE ICDB/95616327
Anonymous; No affiliation given

Abstract: Gene therapy could be used not only to treat single gene defects, but also for many other disorders such as cancer and degenerative neurologic disease. Early attempts at gene therapy involved the indirect approach of modifying cells in culture and then transplanting them back in the body, but this book focuses on direct introduction of genes into the body without cell transplantation. Contributions from 47 authors are organized into three sections. The first provides a background for the concepts of gene therapy, with chapters on history of gene transfer, producing mouse genetic models for human diseases, post-transcriptional considerations for gene expression, and promoters, enhancers, and inducible elements for gene therapy. Section II covers methods and mechanisms with the chapter topics: mechanisms of DNA uptake in skeletal muscle; receptor-mediated gene delivery employing adenovirus-polylysine-DNA complexes; gene transfer using liposomes; in vivo receptor-mediated, and calcium phosphate-mediated DNA transfection; cellular internalization of oligodeoxynucleotides; particle bombardment--the Accell gene gun; and electrotransfection in vivo. The final section on applications covers: pharmacokinetic considerations; virus-mediated genetic treatment of rodent gliomas; gene therapy of adenosine deaminase deficiency and malignant solid tumors; herpes simplex viral vectors for the central nervous system; applications of direct and catheter-based delivery to cardiovascular diseases and malignancy; gene therapy of arthritis; gene therapy: the advent of adenovirus; gene transfer into the heart; use of asialoglycoprotein-polylysine conjugates; and retrovirus-mediated gene transfer and Duchenne muscular dystrophy. There is an authors' keyword index.

Keywords: Adenoviruses, Human Animal Brain Neoplasms/GENETICS/THERAPY Cardiovascular Diseases/THERAPY DNA, Neoplasm/METABOLISM *Gene Therapy *Gene Transfer Genetic Vectors Glioma/GENETICS/THERAPY Human Rodentia Severe Combined Immunodeficiency/THERAPY Simplexvirus/GENETICS MONOGRAPH
951230
M95C3239

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1995. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1995. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .