Diminished V beta T cell subset responses to superantigen in HIV+ individuals as determined by multiparameter flow cytometry.

Important note: Information in this article was accurate in 1995. The state of the art may have changed since the publication date.

Diminished V beta T cell subset responses to superantigen in HIV+ individuals as determined by multiparameter flow cytometry.

Natl Conf Hum Retroviruses Relat Infect (2nd). 1995 Jan 29-Feb 2;:86. Unique Identifier : AIDSLINE AIDS/95920206
Maino V; Ruitenberg JJ; Suni MA; Mole L; Holodniy M; Becton Dickinson Immunocytometry Systems, San Jose, CA.

Abstract: Staphylococcal enterotoxins represent a class of superantigens characterized by an ability to stimulate subsets of T cells expressing unique families of T cell receptor beta chains (TCR V beta) in a manner that is dependent on class II MHC. We have recently developed a multiparameter flow cytometric method for rapidly assessing individual lymphocyte subset responses to a variety of stimuli including superantigen. The assay was utilized to monitor the expression of an early activation marker (CD69) in whole blood samples after 4 hour incubation with superantigens with specificities for T cell subsets expressing a range of TCR V beta variable region families. In whole blood samples obtained from normal donors staphylococcal enterotoxin B (SEB) stimulated CD69 expression on V beta 6 expressing CD4+ T cells but did not stimulate T cells expressing V beta 8 consistent with the specificity reported for this superantigen. In an effort to explore superantigen responses in HIV+ individuals we analyzed CD4+ and CD8+ T cell subsets in normal and seropositive donors for the expression of CD69 following stimulation with SEB using 3-color flow cytometry. Percent positive CD69 expression on specific T cell subsets was related to stage of disease (CD4 counts) and various clinical parameters including identified opportunistic infections. Preliminary studies indicated that a number of samples from HIV+ individuals exhibited significantly diminished responses to SEB compared to normal controls as determined by percentage of CD4+ and CD8+ T cells expressing CD69. This difference was most apparent within the CD8+ T cell population. Longitudinal studies in HIV subjects are currently in progress to examine individual V beta T cell subset responses to superantigen.

Keywords: Flow Cytometry Human HIV Seropositivity/*IMMUNOLOGY Superantigens/*IMMUNOLOGY *T-Lymphocyte Subsets ABSTRACT
951230
M95C2931

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1995. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1995. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .