Cytogenetic changes of human T-lymphotropic virus type I infected human lymphocytes transformed by carcinogen treatment (Meeting abstract).

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Cytogenetic changes of human T-lymphotropic virus type I infected human lymphocytes transformed by carcinogen treatment (Meeting abstract).

2nd International Conference on Gene Regulation/Oncogenesis/AIDS, September 23-30, 1993, Loutraki, Greece, p. 38, 1993.. Unique Identifier : AIDSLINE ICDB/95606698
Fukushima T; Mochizuki S; Kawamura K; Koshikawa N; Wang S; Miyauchi M; Maruyama K; Chiba Cancer Center Research Inst., Chiba, Japan

Abstract: A number of chromosomal abnormalities have been documented in ATL in which human T-lymphotropic virus type I (HTLV-I) has been implicated. In order to investigate the mechanism of transformation of HTLV-I infected cells, lymphocytes from an HTLV-I seronegative normal person were infected in vitro with HTLV-I. A continuous T cell line (Coculture-5) dependent on IL-2 for growth was established. Following treatment with MNNG and TPA, a subline (MNNG-TPA) dependent of TPA for growth was obtained. A subline (MNNG-I) of autonomously growing transformed cells was established following MNNG treatment of Coculture-5 cells. Other transformed cell lines (UV-1, -2, -3, -4, -5) were obtained following ultraviolet ray irradiation of Coculture-5 cells. Karyotype analyses revealed structural abnormalities characteristic of each line clustering to chromosomes 1 and 7, in addition to common abnormalities of chromosome 1 and 8. DNA of these cells subjected to RFLP analyses showed alterations in EcoRI fragments of met gene that is known to reside in chromosome 7, and to be associated with MNNG-induced transformation. Results of PCR-SSCP using specific primers for met gene suggested met gene alterations in cells transformed following treatment with MNNG. Results of ABC tests using specific antibodies indicated changes in expression of proteins coded by genes, including met residing in chromosomes with specific abnormalities, as well as those coded by genes related to the cell cycle including PCNA. Particular cytogenetic changes of HTLV-I infected cells appeared to be involved in their transformation following exposure to carcinogens.

Keywords: Carcinogens/*TOXICITY Cell Division Cell Transformation, Neoplastic Chromosomes, Human, Pair 7 Human *HTLV-I Lymphocytes/*VIROLOGY Methylnitronitrosoguanidine/*TOXICITY Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Tetradecanoylphorbol Acetate/*TOXICITY ABSTRACT
950430
M9541156

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