Trichosanthin, a ribosome inactivating and anti-HIV protein, does not alter the concentration of beta-endorphin in the mouse brain. NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

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Trichosanthin, a ribosome inactivating and anti-HIV protein, does not alter the concentration of beta-endorphin in the mouse brain.

Biochem Mol Biol Int. 1993 Dec;31(6):1101-4. Unique Identifier : AIDSLINE MED/94251174
Ng TB; Kwong WH; Yeung HW; Department of Biochemistry, Faculty of Medicine, Chinese; University of Hong Kong.

Abstract: Trichosanthin was administered intramuscularly into mature male ICR mice at a dosage of 0.2mg/25g, daily for three days. The mice were sacrificed four hours after the last injection. Their brains were dissected out and divided into three regions: A(thalamus and hypothalamus), B(cerebral cortex) and C(cerebellum and brainstem). Their pituitaries were also removed. The pituitaries and the various brain regions were extracted with hot 1M acetic acid. The extracts were lyophilized and reconstituted in radioimmunoassay (RIA) buffer before assay for beta-endorphin by RIA. It was found that there was a significant elevation of beta-endorphin concentration after trichosanthin treatment only in brain region A. The data suggest that trichosanthin does not adversely affect the brain.
Keywords: beta-Endorphin/*METABOLISM Animal Antiviral Agents/PHARMACOLOGY Brain Chemistry/*DRUG EFFECTS Hypothalamus/DRUG EFFECTS/METABOLISM HIV/DRUG EFFECTS Male Mice Mice, Inbred ICR Pituitary Gland/DRUG EFFECTS/METABOLISM Radioimmunoassay Ribosomes/DRUG EFFECTS Thalamus/DRUG EFFECTS/METABOLISM Trichosanthin/*PHARMACOLOGY JOURNAL ARTICLE

KWDbeta-endorphin/KWDmetabolismanimalantiviralagents/pharmacologybrainchemistry/KWDdrugeffectshypothalamus/drugeffects/metabolismhiv/drugeffectsmalemicemice,inbredicrpituitarygland/drugeffects/metabolismradioimmunoassayribosomes/drugeffectsthalamus/drugeffects/metabolismtrichosanthin/KWDpharmacologyjournalarticle
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