Conserved sequence homology of cysteine rich regions in genes encoding glycoprotein A in Pneumocystis carinii derived from different host species.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Conserved sequence homology of cysteine rich regions in genes encoding glycoprotein A in Pneumocystis carinii derived from different host species.

Abstr Gen Meet Am Soc Microbiol. 1994;94:141 (abstract no. D-253). Unique Identifier : AIDSLINE ASM94/94313065
Wright T; Haidaris P; Gigliotti F; Harmsen A; Haidaris C; University of Rochester, NY.

Abstract: Pneumocystis carinii (Pc) surface glycoprotein A (gpA) exhibits host-species specific phenotypic and genotypic variation. Despite this heterogeneity, the gpAs of Pc isolated from different host species appear to be homologous molecules sharing certain biochemical and antigenic characteristics. Using two degenerate oligodeoxyribonucleotide primers corresponding to conserved Cys regions from ferret and rat Pc gpA, a polymerase chain reaction (PCR) product of approximately 300 bp was amplified from ferret, rat, and SCID mouse Pc-infected lung genomic DNA. Northern hybridization revealed a transcript of 3450 nucleotides in Pc-infected SCID mouse lung mRNA, which is similar in size to the transcripts for ferret and rat Pc gpA. Nucleotide sequence analysis of SCID mouse P. carinii gpA subclones derived from the PCR products identified two isoforms, which were 89% identical to each other in the amplified region, and 73% and 54% identical to the rat and ferret derived Pc gpA genes, respectively. Comparison of the deduced amino acid sequences of mouse, ferret and rat Pc gpAs revealed striking similarity in residues adjacent to and including the conserved Cys. Furthermore, the spacing of two Pro residues is invariant, and a potential N-linked glycosylation site is found at a similar position in all of the gpAs. Despite the heterogeneity observed in Pc gpA, the conservation of Cys residues and adjacent sequences implies similar secondary structure and, therefore, function for the gpAs of Pc isolated from different host species.

Keywords: Animal Base Sequence Blotting, Northern Comparative Study *Conserved Sequence Cysteine Ferrets Fungal Proteins/BIOSYNTHESIS/*GENETICS *Genes, Fungal Lung/MICROBIOLOGY Membrane Glycoproteins/BIOSYNTHESIS/*GENETICS Mice Mice, SCID Pneumocystis carinii/*GENETICS/ISOLATION & PURIF Polymerase Chain Reaction/METHODS Proline Rats RNA, Messenger/BIOSYNTHESIS ABSTRACT
941030
M94A0889

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