Genetic analysis of Mycobacterium avium superoxide dismutase. NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

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Genetic analysis of Mycobacterium avium superoxide dismutase.

Abstr Gen Meet Am Soc Microbiol. 1994;94:176 (abstract no. U-21). Unique Identifier : AIDSLINE ASM94/94313071
Escuyer V; Haddad N; Berche P; Laboratoire de Microbiologie, INSERM, Faculte de Medecine; Necker-Enfants Malades, Paris, France.

Abstract: Mycobacterium avium is a major opportunistic pathogen widely spread in the environment and responsible for severe infections in immunocompromised patients with AIDS. It is able to survive and replicate in the phagocytic cells of the host immune system and resists the oxidative burst of activated macrophages. M. avium produces a 23-kDa superoxide dismutase (SOD) that is thought to be involved in the resistance to the respiratory burst-associated bactericidal mechanisms. In order to investigate the molecular basis of this resistance, we have cloned the gene encoding the M. avium SOD by screening a recombinant DNA library with a probe corresponding to the SOD gene from Mycobacterium tuberculosis. The complete nucleotide sequence of the gene was determined. It encodes a 207 amino acid protein that shows a very high homology with the SOD's from Mycobacterium leprae and Mycobacterium tuberculosis (90% and 80% respectively) and very significant homology with manganese or iron-containing SOD's from other organisms including eucaryotes. The exact role of the M. avium SOD in intracellular survival is now under investigation.
Keywords: Amino Acid Sequence AIDS-Related Opportunistic Infections/IMMUNOLOGY/MICROBIOLOGY Comparative Study *Genes, Bacterial Human Macrophages/PHYSIOLOGY Mycobacterium avium/*ENZYMOLOGY/GENETICS Mycobacterium leprae/ENZYMOLOGY/GENETICS Mycobacterium tuberculosis/ENZYMOLOGY/GENETICS Phagocytosis Sequence Homology, Amino Acid Superoxide Dismutase/*GENETICS Tuberculosis/IMMUNOLOGY/MICROBIOLOGY ABSTRACTKWDaminoacidsequenceaids-relatedopportunisticinfections/immunology/microbiologycomparativestudyKWDgenes,bacterialhumanmacrophages/physiologymycobacteriumavium/KWDenzymology/geneticsmycobacteriumleprae/enzymology/geneticsmycobacteriumtuberculosis/enzymology/geneticsphagocytosissequencehomology,aminoacidsuperoxidedismutase/KWDgeneticstuberculosis/immunology/microbiologyabstract
941030
M94A0883

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