Isolation of fastidious mycobacteria from patients with AIDS.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Isolation of fastidious mycobacteria from patients with AIDS.

Abstr Gen Meet Am Soc Microbiol. 1994;94:180 (abstract no. U-45). Unique Identifier : AIDSLINE ASM94/94313072
Yajko DM; Clancy MN; Goodman C; Hadley WK; Univ. of California, San Francisco General Hospital.

Abstract: During a recent 14 month period (6/92-8/93) slow-growing fastidious mycobacteria were recovered at San Francisco General Hospital from 22 specimens submitted from 10 patients with AIDS. Twenty of the isolates (from 9 patients) were recovered from blood and two isolates (from a single patient) were recovered from AFB smear positive sputum specimens. Nineteen of the blood isolates (from 8 patients) grew in Bactec 12B broth with a median time to detection of 42 days (range 14-54 days). Three isolates (a blood isolate from one patient and the two sputum isolates from another patient) did not grow in Bactec 12B broth but were seen on Middlebrook 7H11 agar after 49-54 days of incubation. None of the isolates grew on Lowenstein-Jensen medium but in most cases colonies were seen on the primary non-selective 7H11 agar plate or on the first sub-culture from 12B broth to 7H11 agar. Further sub-culture to solid media (7H11 agar, 7H11 with 5% human blood, 7H10 with mycobactin-J, chocolate agar) was often unsuccessful in our laboratory but growth usually occurred by sub-culturing to 7H9 broth. Isolates from 4 of the patients have been identified by the California State Dept of Health as M. genavense. Identification of the isolates from the remaining patients is still in progress. The results of this study indicate that isolation of fastidious mycobacteria from our patient population is not uncommon and that mycobacterial blood cultures from patients with AIDS should be held for > 6 weeks since one-half of the fastidious isolates were recovered after 42 days of incubation.

Keywords: AIDS-Related Opportunistic Infections/BLOOD/*MICROBIOLOGY Human Mycobacterium/CLASSIFICATION/GROWTH & DEVELOPMENT/*ISOLATION & PURIF Sputum/MICROBIOLOGY Tuberculosis/BLOOD/COMPLICATIONS/*MICROBIOLOGY ABSTRACT
941030
M94A0882

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