Induction of immune mediators by defined lipid fractions from Mycobacterium avium serovar 8. NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

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Induction of immune mediators by defined lipid fractions from Mycobacterium avium serovar 8.

Abstr Gen Meet Am Soc Microbiol. 1994;94:190 (abstract no. U-102). Unique Identifier : AIDSLINE ASM94/94313074
Barrow W; Davis T; Wright E; Labrousse V; Bachelet M; Rastogi N; Institut Pasteur, Paris, France.

Abstract: Because of increasing evidence that M. avium lipids can modulate host responses, lipid fractions from M. avium serovar 8 were evaluated for their ability to modulate the immune function of human peripheral blood mononuclear cells. A total lipid fraction was obtained by the Folch extraction procedure and processed by column chromatography to derive individual fractions which were defined by the presence of glycopeptidolipids (GPL) or related components, and the amount of total carbohydrate and 6-deoxyhexose, as determined by the Dubois and Dische method, respectively. Total lipid and lipid fractions were assessed for their ability to induce prostaglandin E2 (PGE2) and tumor necrosis factor-alpha (TNF-alpha) release and their ability to affect macrophage function as determined by intracellular growth experiments using an unrelated strain of M. avium. All fractions affected human monocyte function, with the more apolar fractions having the most adverse effect. Only the total lipid and GPL fractions were able to induce significant release of TNF-alpha and PGE2. Effectiveness for lipid fractions to be immunomodulatory appeared to be dependent upon the amount of carbohydrate, particularly with regard to 6-deoxy-hexose content, suggesting the importance of GPL and related components in the immune response to M. avium.
Keywords: Dinoprostone/BIOSYNTHESIS Human Lipids/*IMMUNOLOGY/ISOLATION & PURIF Lymphocytes/*IMMUNOLOGY Macrophages/*IMMUNOLOGY/MICROBIOLOGY Mycobacterium avium/CLASSIFICATION/GROWTH & DEVELOPMENT/ *IMMUNOLOGY Serotyping Tumor Necrosis Factor/BIOSYNTHESIS ABSTRACTKWDdinoprostone/biosynthesishumanlipids/KWDimmunology/isolation&puriflymphocytes/KWDimmunologymacrophages/KWDimmunology/microbiologymycobacteriumavium/classification/growth&development/KWDimmunologyserotypingtumornecrosisfactor/biosynthesisabstract
941030
M94A0880

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