The removal of human immunodeficiency virus (HIV) from fluids containing serum with a 0.04 micron nylon membrane filter.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

The removal of human immunodeficiency virus (HIV) from fluids containing serum with a 0.04 micron nylon membrane filter.

Abstr Gen Meet Am Soc Microbiol. 1994;94:424 (abstract no. Q-205). Unique Identifier : AIDSLINE ASM94/94313079
Oshima K; Comans T; Rowe T; Orloff S; Highsmith A; Ades E; Centers for Disease Control and Prevention, Atlanta, GA.

Abstract: The risks associated with blood and blood products contaminated with human immunodeficiency virus (HIV) has been an ongoing concern in the health care industry. We tested the ability of a 0.04 micron nylon membrane filter to remove human immunodeficiency virus (HIV) from fluid containing serum and characterized the effect of filtration on the concentration of serum proteins. The filters were tested with HIV suspended in Dulbecco's modified minimum essential medium (DMEM) containing 10% fetal bovine serum. Endpoint titrations of infectious virus (ID50) were performed on lymphocyte cultures. Virus concentrations were determined using an enzyme-linked immunoabsorbent assay (ELISA) of the HIV-1 P24 core antigen. In three replicate challenges with 47-mm membrane disks, no virus was detected in the filtrate. The titer reduction was estimated to be greater than 8.5 x 10(2). The concentration of IgM (900,000 Da), IgG (160,000 Da), and albumin (67,000 Da) in pooled human serum was determined by ELISA before and after 0.04 micron nylon membrane filtration. High recovery efficiency of these proteins were observed. No relationship between the molecular weight of the protein and recovery was detected. The results suggest that the pore size distribution of the 0.04 micron membrane filter is sufficient to allow biologically active serum proteins through during filtration.

Keywords: Blood/*MICROBIOLOGY Blood Proteins/ANALYSIS Enzyme-Linked Immunosorbent Assay Human HIV/*ISOLATION & PURIF HIV Core Protein p24/ANALYSIS HIV-1/*ISOLATION & PURIF IgM/BLOOD Nylons Risk Factors Serum Albumin/ANALYSIS Ultrafiltration/*METHODS ABSTRACT
941030
M94A0875

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