Identification of three distinct HIV-1 subtypes in Brazil based on the protease gene.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Identification of three distinct HIV-1 subtypes in Brazil based on the protease gene.

Abstr Gen Meet Am Soc Microbiol. 1994;94:482 (abstract no. T-1). Unique Identifier : AIDSLINE ASM94/94313081
Janini LM; Luo CC; Peralta M; Schecther M; Kalish ML; Pieniazek N; de la Torre N; Schochetman G; Rayfield M; Pieniazek D; CDC, Atlanta, GA.

Abstract: OBJECTIVE: To study genotypic differences in the protease gene from HIV-1 infected persons in Brazil. METHODS: HIV-1 proviral DNA from 40 specimens were surveyed for genetic similarity to reference strains using a PCR typing procedure based on the polymorphism of restriction sites in the p24 gag region and in the protease gene. Twenty four of these isolates were selected for protease gene sequencing. The sequence data were used to infer phylogenetic relationship between isolates using maximum likelihood and distance matrix methods. RESULTS: Three genetically distinct subtypes were identified on the basis of their protease gene sequences. Twenty one of the sequences were related to those of the North American/European env subtype (B). The remaining 3 strains clustered into 2 distinct groups in the phylogenetic tree: one sequence clustered with the African strains ELI and Z2Z6 (env subtype D), whereas two other sequences clustered with strains in the env subtype F group. CONCLUSIONS: Analysis of protease gene demonstrated the existence of 3 distinct HIV-1 phylogenetic groups corresponding to env subtypes B, D and F in the Brazilian population in contrast to the two env subtypes (B and F) previously identified. Whether differences between the grouping of some Brazilian strains, based on protease or envelope gene regions, is a result of convergence in V3 region or a recombination event merits further studies.

Keywords: Brazil Comparative Study DNA, Viral/*ISOLATION & PURIF Genes, gag *Genes, Viral Human HIV Protease/*GENETICS HIV-1/*CLASSIFICATION/*GENETICS/ISOLATION & PURIF Phylogeny Polymerase Chain Reaction/METHODS Polymorphism (Genetics) ABSTRACT
941030
M94A0873

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