A rapid sensitive, solution based fluorescent in situ hybridization (FISH) assay for the detection of HIV mRNA in infected cell lines by multi-colored flow cytometry.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

A rapid sensitive, solution based fluorescent in situ hybridization (FISH) assay for the detection of HIV mRNA in infected cell lines by multi-colored flow cytometry.

Abstr Gen Meet Am Soc Microbiol. 1994;94:483 (abstract no. T-3). Unique Identifier : AIDSLINE ASM94/94313084
Poindexter B; Cubbage M; Weber W; Ku J; Bresser J; Aprogenex, Inc., Houston, TX.

Abstract: Rapid detection of low levels of HIV infected cells is necessary for the screening of donated blood as well as in the clinical diagnosis of infection. Aprogenex has developed an HIV diagnostic test, Virafy, sensitive enough to detect infected cell lines diluted down to 1 in 10,000 with uninfected cell lines by flow cytometry. The test can be performed in less than 1 hour and with the use of proprietary background reducers and signal enhancers these low levels of detection can be obtained. Other potential applications include determination of the level of RNA infection in cells as well as the evaluation of therapeutic regimens.

Keywords: Flow Cytometry/*METHODS Human HIV/GENETICS/*ISOLATION & PURIF In Situ Hybridization, Fluorescence/*METHODS Reagent Kits, Diagnostic RNA, Messenger/*ANALYSIS Sensitivity and Specificity ABSTRACT
941030
M94A0870

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