The upregulation of the promoter gene encoding the p105/p50 subunit of NF-kB by HIV is dependent upon one specific kB motif.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

The upregulation of the promoter gene encoding the p105/p50 subunit of NF-kB by HIV is dependent upon one specific kB motif.

Abstr Gen Meet Am Soc Microbiol. 1994;94:483 (abstract no. T-5). Unique Identifier : AIDSLINE ASM94/94313088
Ten R; MacMorran W; Rolli V; Israel A; Paya C; Division of Experimental Pathology, Mayo Clinic, Rochester, MN; 55905.

Abstract: Our previous cloning and sequencing of the promoter of the gene encoding the p50 subunit of NF-kB indicated that it is upregulated by NF-kB. Furthermore, we have shown that in persistently HIV-infected monocytic cells, which contain increased NF-kB activity, the transcriptional regulation of this promoter is increased. We have further characterized the different regulatory sequences of the p105/p50 promoter that could potentially participate in its autoregulation. One AP-1, four NF-kB like and one HIP-1 motifs are present in this promoter. Gel shift analysis using nuclear extracts from monocytic cells unstimulated or treated with LPS or HIV-infected demonstrated specific binding proteins to the AP-1 site, to the three of the four kB sites, but not to the HIP-1 motif. One of the three kB sites (kB4) bound NF-kB complexes with higher affinity than the rest. Deletion and mutation constructs of the p105 promoter cloned upstream a luciferase reporter gene followed by transfection into monocytic cells was performed. The AP-1, kB1, kB2, kB3, and HIP-1 regions were found to be dispensable for the basal and LPS-inducible transcriptional activity of the promoter in uninfected and HIV-infected monocytes. Only the kB4 site was identified as essential in the regulation of the p105 promoter. These results suggest that the p105/p50 promoter is solely regulated by NF-kB complexes binding with high affinity to only one of the kB motifs in monocytic cells.

Keywords: Binding Sites *Gene Expression Regulation, Viral Human HIV/*GENETICS Lipopolysaccharides/PHARMACOLOGY Luciferase/BIOSYNTHESIS Monocytes/DRUG EFFECTS/*METABOLISM/MICROBIOLOGY NF-kappa B/BIOSYNTHESIS/*GENETICS/METABOLISM *Promoter Regions (Genetics) Recombinant Proteins/BIOSYNTHESIS Transcription, Genetic Transfection ABSTRACT
941030
M94A0866

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1994. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1994. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .