Feline bone marrow stromal fibroblasts are a target of feline immunodeficiency virus infection in vitro and in vivo.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Feline bone marrow stromal fibroblasts are a target of feline immunodeficiency virus infection in vitro and in vivo.

Abstr Gen Meet Am Soc Microbiol. 1994;94:485 (abstract no. T-18). Unique Identifier : AIDSLINE ASM94/94313095
Lu YS; Ho SI; Read RW; Richardson JA; Pakes SP; University of Texas Southwestern Medical Center, Dallas.

Abstract: Similar to HIV infection in humans, cats infected with feline immunodeficiency virus (FIV) develop anemia, neutropenia and lymphopenia. These hematologic abnormalities are thought to be due to altered marrow micro-environments because of infected marrow stromal cells. Susceptibility of marrow stromal cells to FIV infection is unknown. Stromal fibroblasts from bone marrow cells isolated from three SPF cats were grown in culture medium and were exposed to FIV (Petaluma strain). Portions of the FIV-exposed stromal fibroblasts were cocultured with CRFK cells to amplify FIV infection. Culture supernatants and cells from amplified and nonamplified cultures were assayed for viral infection. Results show that cells from both cultures are positive for proviral DNA by PCR and viral antigens by IFA. However, p26 viral antigen (ELISA assay) is found only in supernatant of stromal fibroblasts cocultured with CRFK cells. Similarly, marrow stromal fibroblasts from three FIV-infected cats were prepared and treated in the same manner without exposure to FIV in vitro. Some of the stromal fibroblasts were cocultured with CRFK cells or Con A-activated peripheral blood lymphoblasts from an SPF cat. The results show that proviral DNA and viral antigens are positive in stromal fibroblasts and cocultured cells. However, p26 viral antigen is detected only in supernatant of stromal fibroblasts of individual cat cocultured with CRFK or activated lymphoblasts, but not both. Both in vitro and in vivo results indicate that feline marrow stromal fibroblasts are a target of FIV infection. FIV-infected cats may serve a good model to study hematopoietic dysfunction of HIV infection in humans.

Keywords: Animal Antigens, Viral/ANALYSIS Bone Marrow/*CYTOLOGY/MICROBIOLOGY Cats Cells, Cultured Comparative Study DNA, Viral/ANALYSIS Enzyme-Linked Immunosorbent Assay Feline Acquired Immunodeficiency Syndrome/*PHYSIOPATHOLOGY Fibroblasts/CYTOLOGY/MICROBIOLOGY Hematopoiesis Human HIV Infections/PHYSIOPATHOLOGY Immunodeficiency Virus, Feline/*GROWTH & DEVELOPMENT/GENETICS/ ISOLATION & PURIF Polymerase Chain Reaction Proviruses/GROWTH & DEVELOPMENT/GENETICS/ISOLATION & PURIF ABSTRACT
941030
M94A0859

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