Peripheral blood and milk-derived leukocytes harbor BIV provirus. NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

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Peripheral blood and milk-derived leukocytes harbor BIV provirus.

Abstr Gen Meet Am Soc Microbiol. 1994;94:486 (abstract no. T-22). Unique Identifier : AIDSLINE ASM94/94313098
Nash JW; Hanson LA; St. Cyr Coats K; Mississippi State University.

Abstract: Bovine immunodeficiency virus (BIV), a lentivirus, infects a large population of cattle in the southern United States. The mode of transmission of BIV is unknown, but its similarity to HIV suggests analogous mechanisms of transmission. The purpose of this study was to develop a PCR probe to detect BIV proviral sequences in cells which are likely vehicles for BIV transmission. DNA was prepared from peripheral blood (PBL) and milk-derived leukocytes and used as template for PCR. The proviral target for PCR amplification was a 234 bp fragment within a highly conserved region of the BIV pol gene. DNA fragments of approximately 234 bp were amplified from the DNA of both PBL and milk-derived leukocytes from BIV seropositive animals. Nucleotide sequencing of the amplified products confirmed their BIV specificity. Further study is necessary to determine the role of whole blood and milk in transmission of BIV in natural infections.
Keywords: Animal Base Sequence Cattle Cattle Diseases Comparative Study Conserved Sequence DNA, Viral/*ANALYSIS/BLOOD Female Genes, pol Immunodeficiency Virus, Bovine/GENETICS/*ISOLATION & PURIF Lentivirus Infections/DIAGNOSIS/VETERINARY Leukocytes/*MICROBIOLOGY Milk/*MICROBIOLOGY Polymerase Chain Reaction/METHODS Proviruses/GENETICS/ISOLATION & PURIF ABSTRACTKWDanimalbasesequencecattlecattlediseasescomparativestudyconservedsequencedna,viral/KWDanalysis/bloodfemalegenes,polimmunodeficiencyvirus,bovine/genetics/KWDisolation&puriflentivirusinfections/diagnosis/veterinaryleukocytes/KWDmicrobiologymilk/KWDmicrobiologypolymerasechainreaction/methodsproviruses/genetics/isolation&purifabstract
941030
M94A0856

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