Evaluation of a human immunodeficiency virus-1 antibody immunoblot for the confirmation of oral specimens.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Evaluation of a human immunodeficiency virus-1 antibody immunoblot for the confirmation of oral specimens.

Abstr Gen Meet Am Soc Microbiol. 1994;94:622 (abstract no. V-29). Unique Identifier : AIDSLINE ASM94/94313118
Griffin CK; Chun JK; Allen NM; Gaudette ND; Erbling MC; Jacobs AL; Goldstein AS; Cimler BM; Epitope, Inc., Beaverton, Oregon.

Abstract: Western blot (WB) assays are considered the gold standard for confirming retroviral screening test results. Specimens obtained with the OraSure oral specimen collection device have been extensively tested for the detection of antibodies to HIV and other viral infections. An HIV-1 WB was developed for the confirmation of the immunostatus of OraSure specimens. To evaluate the performance of this WB, paired serum and oral samples collected from 226 seropositive and 204 seronegative subjects were tested. The serum specimens were tested using an FDA-licensed HIV-1 WB kit; the matching OraSure specimens were tested using the OraSure WB assay. Among the 226 confirmed seropositive samples, the OraSure HIV-1 WB was positive for 224 samples and indeterminate for the remaining two. No false negative samples were observed. None of the 204 specimens identified as HIV-1 non-reactive by EIA testing was found to be positive by either the OraSure HIV-1 WB or the licensed serum WB.

Keywords: Acquired Immunodeficiency Syndrome/BLOOD/*DIAGNOSIS/PREVENTION & CONTROL Antibodies, Viral/*ANALYSIS/BLOOD Comparative Study Human *HIV Seronegativity HIV Seropositivity/BLOOD/*DIAGNOSIS HIV-1/*IMMUNOLOGY/ISOLATION & PURIF Immunoenzyme Techniques Mass Screening/METHODS Mouth Mucosa/IMMUNOLOGY/*MICROBIOLOGY Predictive Value of Tests Specimen Handling/METHODS ABSTRACT
941030
M94A0836

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