Detection of JC virus DNA by PCR in brain tissue and cerebrospinal fluid from HIV infected patients.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Detection of JC virus DNA by PCR in brain tissue and cerebrospinal fluid from HIV infected patients.

Abstr Gen Meet Am Soc Microbiol. 1994;94:623 (abstract no. V-36). Unique Identifier : AIDSLINE ASM94/94313123
Caldarelli Stefano R; Omodeo Zorini E; Rivolta GE; Achilli G; Cattaneo E; Vago L; Boldorini R; Ferrante P; Biology Lab., Fondazione Don C. Gnocchi IRCCS, Milano, Italy.

Abstract: The correct diagnosis of progressive multifocal leukoencephalopathy (PML), a severe demyelinating disease caused from JC Virus, is of particular relevance in AIDS patients. For these reasons we set a Polymerase Chain Reaction (PCR) method to detect JCV DNA both in brain tissues collected at autopsy from 17 cases and in cerebrospinal fluid (CSF) and urine of 7 AIDS patients with different neurological symptoms, including a suspected PML case. The genomic region chosen for PCR belongs to the Large T antigen of JCV and contains a Bam HI site that is present only in JCV but not in BKV genome. JCV DNA has been detected in 7 (58.3%) paraffin-embedded, in 2 (66.6%) formalin-fixed, in 2 (100%) frozen tissues and in 2 (100%) frozen CSF. Regarding the 7 neurologically ill patients only one, presenting clinical and radiological PML signs, was positive both in CSF and urine for JCV DNA. Our results indicate that PCR is useful in the diagnosis of PML at autopsy but mainly that it is efficient, specific and practical for the correct diagnosis and follow-up of living AIDS patients.

Keywords: Autopsy AIDS-Related Opportunistic Infections/CEREBROSPINAL FLUID/ *DIAGNOSIS/URINE Brain/*MICROBIOLOGY DNA, Viral/*ANALYSIS/CEREBROSPINAL FLUID/URINE Follow-Up Studies Human Papovaviridae Infections/COMPLICATIONS/*DIAGNOSIS Polymerase Chain Reaction/*METHODS Polyomavirus hominis 2/*ISOLATION & PURIF Sensitivity and Specificity Tumor Virus Infections/COMPLICATIONS/*DIAGNOSIS ABSTRACT
941030
M94A0831

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1994. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1994. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .