Quantitative determination of HIV-1 p24 antigen in HIV antibody positive sera following alkaline dissociation of immune complexes.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Quantitative determination of HIV-1 p24 antigen in HIV antibody positive sera following alkaline dissociation of immune complexes.

Abstr Gen Meet Am Soc Microbiol. 1994;94:624 (abstract no. V-37). Unique Identifier : AIDSLINE ASM94/94313126
Swack N; Ramirez M; Berberich S; Stapleton J; Hygienic Laboratory, Univ. of Iowa, Iowa City.

Abstract: Assays for the detection of HIV-1 antigen have been used for the detection of circulating virus early in infection and also for assessment of disease progression and response to antiviral therapy. However HIV-1 p24 antigen may be complexed with HIV antibody and thus escape detection by antigen capture immunoassay. Previous studies to assay virus after acid dissociation of the immune complexes was found to be time consuming and required large quantities of serum. These problems were significantly reduced by the use of alkaline dissociation which was significantly less labor intensive and required smaller amounts of serum. Ninety seven serum specimens obtained from patients at the University of Iowa AIDS Clinic were studied. All sera were found to be HIV-1 antibody positive by Western Blot analysis. The semiquantitative detection of p24 antigen was studied by antigen capture assays obtained from commercial sources (Abbott Laboratories and Organon Teknika). Antigen dissociation was performed with the Vironostika HIV-1 Antigen Base Dissociation Assay. Both antigen assays without dissociation found 35% of the sera to be positive for the presence of p24 antigen. Following treatment with the alkaline dissociation reagents 67% of the same sera were positive for the presence of p24 antigen. All of these could be neutralized by specific antibody to HIV. Quantitative p24 determinations performed by both the undissociation and alkaline dissociation procedures were compared with patient lymphocyte immunophenotyping studies.

Keywords: Acquired Immunodeficiency Syndrome/*BLOOD/IMMUNOLOGY Antigen-Antibody Complex/*BLOOD/CHEMISTRY/ISOLATION & PURIF Blotting, Western/METHODS Comparative Study Human HIV Antibodies/*BLOOD HIV Antigens/BLOOD HIV Core Protein p24/*BLOOD HIV-1/IMMUNOLOGY/*ISOLATION & PURIF Immunophenotyping/METHODS Lymphocytes/IMMUNOLOGY Reagent Kits, Diagnostic ABSTRACT
941030
M94A0828

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