DDD/1-Mtv-2/Mtv-2 congenic mice (2): immunological characterization (Meeting abstract). NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

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DDD/1-Mtv-2/Mtv-2 congenic mice (2): immunological characterization (Meeting abstract).

International Association for Breast Cancer Research, Biennial Meeting. April 25-28, 1993, Calgary, Alberta, Canada, A31, 1993.. Unique Identifier : AIDSLINE ICDB/94699092
Matsuzawa A; Takeda Y; Sayama K; Nakano H; Laboratory Animal Research Center, Yokohama, Japan

Abstract: DDD/1 (DDD) mice free from exogenous mouse mammary tumor virus (MMTV) were characterized by congenitally markedly reduced lymph node (LN) cell counts. This characteristic defect was attributed mainly to the marked paucity of CD4+ and CD8+ T cells, approx 30 and 10% of LN cells, respectively. In DDD-Mtv-2/MtY-2 (DDD-Mtv-2) congenics, the numbers of CD4+ and CD8+ cells increased by 4 to 18 times depending on ages but that of B cells doubled at the most. Enhancement of T cell proliferation was of the same extent in Vbeta5+ and Vbeta8+ subsets. The thymus weight also increased evidently around 4 wk of age when it reached a peak in congenics. Thus, Mtv-2 enhanced T cell proliferation in a nonspecific fashion. DDD-Mtv-2 but not DDD mice produced a progressive age-dependent deletion of Vbeta14+CD4+ cells. Mtv-2 also caused deletion of Vbeta14+CD8+ cells in LN. In the thymus Vbeta14+CD4+ mature but not immature thymocytes were deleted by Mtv-2. Thus, Mtv-2-dependent Vbeta14+ cell deletion were considered to result from intrathymic elimination. The I-E gene was not expressed in both DDD and DDD-Mtv-2 mice, indicating that deletion of Vbeta14+ T cells can be induced by Mtv-2 without involvement of I-E molecules. DDD-Mtv-2 mice will provide a unique model for researches into clonal deletion of T cells by superantigens produced by Mtv genes.
Keywords: Animal Antigens, CD4/ANALYSIS *CD4-CD8 Ratio Gene Deletion Integrins/ANALYSIS Lymph Nodes Mammary Neoplasms, Experimental/GENETICS/*IMMUNOLOGY *Mice T-Lymphocytes/CYTOLOGY Tumor Viruses, Murine/GENETICS/*IMMUNOLOGY ABSTRACT

KWDanimalantigens,cd4/analysisKWDcd4-cd8ratiogenedeletionintegrins/analysislymphnodesmammaryneoplasms,experimental/genetics/KWDimmunologyKWDmicet-lymphocytes/cytologytumorviruses,murine/genetics/KWDimmunologyabstract
941130
M94B0787

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