Presence of HTLV-I in astroglial cells infected with cell-free virus preparation (Meeting abstract).

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Presence of HTLV-I in astroglial cells infected with cell-free virus preparation (Meeting abstract).

Chronica Dermatol; 2(3):444-5 1992. Unique Identifier : AIDSLINE ICDB/94696621
Macchi B; Graziani G; Caronti B; Lauro GM; Bonmassar E; Dept. of Experimental Medicine and Biochemical Sciences, Univ.; 'Tor Vergata,' Rome, Italy

Abstract: Previous data have shown that astroglial cells express the virus core protein p19 1 wk after exposure to an HTLV-I donor cell line. In order to investigate whether HTLV-I was able to replicate within astroglial cells, glioma cell lines after 20-40 passages in vitro, were exposed to HTLV-I using a cell-free virus preparation. The virus was prepared by ultracentrifugation of supernatants obtained from the virus-producing cell line MT-2 and added to 0.5 x 10(5) glioma cells seeded in a 96-well tissue-culture plate. The presence of HTLV-I was tested weekly by polymerase chain reaction (PCR) using a primer pair specific for the HTLV-I Pol region, that encodes the reverse transcriptase required for the virus replication. Cells were boiled and crude lysates were used as source of DNA to be subjected to 30 cycles of DNA amplification. Amplified DNA was hybridized in a liquid phase to a 32P end-labeled oligonucleotide probe, corresponding to the region amplified by the primer pair used. The results of these experiments showed the presence of HTLV-I in the glioma cells 1 wk after exposure to the virus. However, the level of the virus DNA decreased progressively after 4 wk in culture. In parallel, the expression of the virus core protein p19 substantially diminished as shown in a table. These data suggest that HTLV-I replicates in astroglial cells, however, astroglial cells do not seem to be a permanent reservoir of the virus. (4 Refs)

Keywords: Animal Brain Neoplasms/*MICROBIOLOGY DNA, Viral/ANALYSIS Glioma/*MICROBIOLOGY HTLV-I/GENETICS/ISOLATION & PURIF/*PHYSIOLOGY HTLV-I Infections/GENETICS/MICROBIOLOGY Neuroglia/*MICROBIOLOGY *Virus Replication ABSTRACT
940330
M9431034

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