Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.
Identification and characterization of novel antigenic determinants and endogenous retroviruses in normal and neoplastic canine lymphoid cells.
Diss Abstr Int [B]; 54(2):685 1993. Unique Identifier : AIDSLINE ICDB/94696659 Jure MN; No affiliation given
Abstract:
Studies were conducted to characterize the canine species as a model of lymphomagenesis. The first objective was to develop monoclonal antibodies against canine lymphocyte surface antigens and apply these reagents in the characterization of canine lymphoid malignancies. Second, since several lines of evidence indicated that endogenous retroviruses were expressed in lymphoid neoplasms and transformed cells, a study designed to identify endogenous retroviruses in the canine genome was undertaken. Several monoclonal antibodies were produced. Monoclonal antibody 2E9 recognized a panleukocyte antigen absent from certain T-cell hyperplasias that appears as a useful reagent for the discrimination between this type of hyperplasia and lymphomas. Monoclonal antibody 8E3 recognized an antigen detected on the surface of lymphoid malignancies, mitogen-stimulated lymphocytes, and transformed cell lines but not on normal resting cells. Monoclonal antibody 7A4 bound to an activation antigen also expressed on immature lymphocytes. Monoclonal antibodies 7A4 and 8E3 appear as useful reagents for the classification, diagnosis and prognosis of canine lymphoid neoplasms. Reverse transcriptase (RT) activity (Mn++ dependent) was detected in the supernatant of a canine transformed cell line. Particles containing RT activity banded in sucrose at a density of 1.15 g/cc. RNA contained in these particles was subjected to a RT-PCR-based procedure using degenerate primers derived from a conserved region of the retroviral polymerase gene. A predicted product of 136 bp was obtained, cloned and sequenced. The amplified product showed 61% homology to the simian type D retrovirus SRV-2 and complete homology with a consensus amino acid sequence derived from known retroviruses. A probe derived from the cloned amplified fragment was used to hybridize canine genomic DNA. Southern blot analysis showed that between 1 and 10 copies of this gene are present in the canine genome. In addition, a canine genomic library was screened with the amplified product. Several different recombinant phages were purified and partially characterized. Restriction enzyme analysis showed that conserved restriction sites in the pol gene were found in the different clones. (Full text available from University Microfilms International, Ann Arbor, MI, as Order No. AAD93-18789)
Keywords: Amino Acid Sequence Animal Antibodies, Monoclonal/DIAGNOSTIC USE Antigens, Neoplasm/*ANALYSIS Cell Transformation, Neoplastic/GENETICS/IMMUNOLOGY Dogs *Epitopes Gene Amplification Lymphocytes/DRUG EFFECTS/IMMUNOLOGY Lymphoma/*CHEMISTRY/DIAGNOSIS Mitogens/PHARMACOLOGY Polymerase Chain Reaction Retroviridae/GENETICS/*ISOLATION & PURIF Retroviruses Type D, Simian/*GENETICS/IMMUNOLOGY Sequence Homology, Amino Acid THESIS 940630
M9460919
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Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.
