TH1/TH2 subset analysis in PBMC samples of sooty mangabeys and rhesus macaques naturally and experimentally infected with SIV.

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TH1/TH2 subset analysis in PBMC samples of sooty mangabeys and rhesus macaques naturally and experimentally infected with SIV.

Symp Nonhum Primate Models AIDS. 1993 Sep 19-22;11:abstract no. 16. Unique Identifier : AIDSLINE PRIM11/94191608
Ansari AA; Mayne A; Hunt D; Villinger F; Dept of Pathology, Emory School of Medicine, Yerkes Reg. Primate; Res. Center, Emory Univ., Atlanta, GA.

Abstract: Differential production of cytokines by T cell subsets during an immune response has been shown to have an important regulatory effect on the nature of the immune response and the eventual clinical outcome for the host. Based on the view that disease resistance vs susceptibility could be secondary to differential frequencies of TH1 vs TH2 type of SIV specific immune response, our laboratory has derived cloned T cell lines from these 2 species. Each cloned T cell line has been subjected to phenotypic analysis and to cytokine message analysis using RT-PCR techniques to determine their profile. Detection of mRNA for IL-2, IL-3, IL-4, IL-10 and IFN gamma allowed for the categorization of clones into TH0 (IL-2, IFN gamma, IL-4, IL-10), TH1 (IL-2, IL-3, IFN gamma, IL-10) or TH2 (IL-2, IL-3, IL-4, IL-10). Of the 56 cloned T cell lines from mangabeys, 15 were typed as TH0, 7 as TH1, but 30 as TH2, 4 could not be classified. In contrast, of the 42 T cell lines from macaques, while 15 were typed as TH0, 22 were typed as TH1, but only 5 were typed as TH2. Of interest was the observation that a high frequency of cloned T cell lines (24/42) from macaques were CD4+/CD8+ (DP), whereas only 3/56 cloned T cell lines from mangabeys were DP. These data suggest that asymptomatic macaques infected with SIV have a high frequency of TH1 type of T cells as compared with a predominant TH2 profile in asymptomatic SIV infected mangabeys. The implication of such differential T cell subset response will be discussed.

Keywords: Animal Cercocebus atys Clone Cells Cytokines/*BIOSYNTHESIS CD4-CD8 Ratio Interferon Type II/BIOSYNTHESIS/BLOOD Interleukins/*BIOSYNTHESIS/BLOOD Macaca mulatta RNA, Messenger/ANALYSIS Simian Acquired Immunodeficiency Syndrome/BLOOD/*MICROBIOLOGY Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. *SIV T-Lymphocyte Subsets/*IMMUNOLOGY T-Lymphocytes/IMMUNOLOGY ABSTRACT
940730
M9470910

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