Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.
Cellular immune responses in rhesus macaques with low dose SIV infection.
Symp Nonhum Primate Models AIDS. 1993 Sep 19-22;11:abstract no. 24. Unique Identifier : AIDSLINE PRIM11/94191617 Salvato MS; Emau P; Malkovsky M; Schultz K; McKenzie D; Johnson E; Pauza CD; University of Wisconsin, Dept. of Pathology and Laboratory; Medicine, Madison.
Abstract:
Ten juvenile rhesus monkeys were infected via the rectal mucosa with doses of SIVmac251 ranging from 0.1 to 1000 animal infectious doses (Pauza et al. J Med Primatol., in press, 1993). The infectious dose (ID) is the minimum intravenous inoculum needed to infect 50% of age and sex-matched animals. Two monkeys were infected intravenously (iv) with 10 ID. The 2 iv-inoculated monkeys seroconverted within 2-3 weeks after inoculation, and 3 of 4 monkeys inoculated intrarectally (ir) with > or = 100 infectious doses seroconverted within 2 months post-infection. In contrast, the animals infected ir with < or = 10 ID have not seroconverted throughout the duration of the study, (over 1 year). Sera were tested by an HIV-2-specific ELISA, by radioimmunoprecipitation, and by indirect immunofluorescence on SIV-infected cells. Cytotoxic and proliferative T cell responses were evaluated for seropositive and seronegative ir-infected animals. MHC-restricted CTL specific for gag peptide were observed in seropositive animals, but they have not yet been observed in seronegative animals. T cell proliferative responses to SIV antigens were detected for all ir-infected animals irrespective of dose or antibody status. Seronegative animals with T-cell proliferative responses to SIV antigens remain clinically well, manifest stable CD4+ T cell counts and CD4+/CD8+ cell ratios, and are persistently positive for SIV DNA in PBMC. The observation of seronegative status in conjunction with virus-specific T-cell proliferative responses indicates a role for cellular immunity that may not include MHC-restricted CTL.
Keywords: Animal Cytotoxicity, Immunologic CD4-CD8 Ratio DNA, Viral/ANALYSIS Enzyme-Linked Immunosorbent Assay Female Fluorescent Antibody Technique Gene Products, gag/IMMUNOLOGY Immunity, Cellular Intestinal Mucosa/MICROBIOLOGY Lymphocyte Transformation Macaca mulatta Male Rectum Simian Acquired Immunodeficiency Syndrome/*IMMUNOLOGY/ TRANSMISSION *SIV/ISOLATION & PURIF T-Lymphocyte Subsets/IMMUNOLOGY T-Lymphocytes/*IMMUNOLOGY ABSTRACT
940730
M9470901
AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.
Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.
