Antiviral cytotoxic responses of primate gamma delta T cells isolated by magnetic cell separation.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Antiviral cytotoxic responses of primate gamma delta T cells isolated by magnetic cell separation.

Symp Nonhum Primate Models AIDS. 1993 Sep 19-22;11:abstract no. 26. Unique Identifier : AIDSLINE PRIM11/94191619
Wallace M; Pauza CD; Malkovsky M; Dept. of Medical Microbiology and Immunology, Univ. of Wisconsin; at Madison.

Abstract: We have previously reported that certain human or rhesus monkey gamma delta T cell clones lyse lymphoid cells infected with immunodeficiency viruses (Malkovsky et al., J. Med. Primatol. 21:113, 1992). The same uninfected lymphoid cells are not lysed. This observation and the report that elevated numbers of gamma delta T cells have been observed in HIV-1 infected individuals (De Paoli et al., Clin. Exp. Immunol. 83:187, 1991) suggest a role for this T lymphocyte subset in AIDS. During our experience in culturing these cells, we have observed increases in both the percentage and absolute number of gamma delta T cells in peripheral blood mononuclear cell populations from both humans and rhesus monkeys cultured in medium with IL-2. As an alternative to gamma delta T cell cloning, we have isolated gamma delta T cells from these cultures using positive selection by monoclonal antibodies and magnetic beads. The functional responses of these cells and gamma delta T cell clones were compared. Initial experiments examining cytotoxic responses of human gamma delta T cells isolated by this method indicate that these gamma delta T cell lines exhibit selective cytotoxicity for immunodeficiency virus-infected cells as do some gamma delta T cell clones. With this method, we may be able to assess the potential antiviral response of the gamma delta T cell population present in immunodeficiency virus-infected primates without the lengthy and laborious procedure of generating gamma delta T cell clones.

Keywords: Acquired Immunodeficiency Syndrome/*IMMUNOLOGY Animal Cell Separation/METHODS Cells, Cultured Clone Cells *Cytotoxicity, Immunologic Human Macaca mulatta Magnetics Primates Receptors, Antigen, T-Cell, gamma-delta/*ANALYSIS T-Lymphocyte Subsets/CYTOLOGY/*IMMUNOLOGY ABSTRACT
940730
M9470899

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