Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.
Dual infection of rhesus macaques with SIVE11s and SIV239.
Symp Nonhum Primate Models AIDS. 1993 Sep 19-22;11:abstract no. 4. Unique Identifier : AIDSLINE PRIM11/94191634 Lewis MG; Bellah S; McKinnon K; Yalley-Ogunro J; Greenhouse J; Elkins WR; Rosenberg Y; Gartner S; Eddy G; Henry M. Jackson Foundation, Rockville, MD.
Abstract:
The purpose for this study was to determine if dual infection by two virulent and pathogenic SIV isolates is possible in rhesus macaques. In addition, we wanted to determine if pre-existing immunity alters the anticipated course of infection. The two isolates used in this study, SIVmac239 and SIVmne/E11s, differ genetically by only 6-7% in the gag or envelope gene. Both isolates have been shown to cause disease, at different rates and intensities, in rhesus macaques. Monkeys infected with either isolate generate cross-reacting antibodies to all of the SIV proteins. Eight rhesus macaques previously infected with SIVmne/E11s were used in this study. These monkeys had circulating SIV antibody of differing levels, had previously been virus isolation positive, but at the time of the second challenge were virus isolation negative. The eight monkeys, plus two controls, were challenged with either 10 ID50 or 1000 ID50 of SIVmac239. Seven of the eight animals became virus isolation positive within 14 day after the 239 challenge, with 4 positive by 7 days post-challenge. All seven had anamnestic responses to SIV antigens by 7 to 21 days PI. The remaining macaque, which had the highest levels of SIV-antibody prior to the second challenge, became virus isolation positive on week 6 PI, but remained so for only 2 weeks. Four of the eight animals became virus isolation negative by 8 weeks, while the remaining 4 were routinely isolation positive. No acute syndrome was detected in any of the animals with pre-existing immunity, whereas both control animals had detectable antigenemia on day 10 PI and had lymphadenopathy within two weeks PI. Preliminary analysis by RT-PCR of virus loads in the peripheral blood indicate that antibody levels prior to the second challenge correlates inversely with detectable virus signal. The PCR analysis indicated that the isolated virus was SIVmac239. These finding show that rhesus macaques with varying levels of pre-existing SIV immunity to SIVmne/E11s can be dually infected with SIVmac239. The level of dual infection and pattern of virus isolation appears to be a function of the level of pre-existing SIV specific immunity, but this immunity is not sufficient to block infection by the second isolated.
Keywords: Animal Antibodies, Viral/*BLOOD Antigens, Viral/IMMUNOLOGY Cross Reactions Genes, env Genes, gag Macaca mulatta Polymerase Chain Reaction Simian Acquired Immunodeficiency Syndrome/BLOOD/IMMUNOLOGY/ *MICROBIOLOGY SIV/GENETICS/ISOLATION & PURIF/*PATHOGENICITY Virulence ABSTRACT 940730
M9470884
AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.
Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.
