Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.
Total lymphoid irradiation of SIV-infected macaques as a potential therapy for HIV infection.
Symp Nonhum Primate Models AIDS. 1993 Sep 19-22;11:abstract no. 41. Unique Identifier : AIDSLINE PRIM11/94191636 Fultz PN; Schwiebert R; Su L; Salter M; Department of Microbiology, University of Alabama at Birmingham; 35294.
Abstract:
Lymph nodes appear to be reservoirs for large amounts of virus in HIV-infected persons. It is also clear that many manifestations of HIV-related disease are due to immune activation and autoimmune reactions. Total lymphoid irradiation (TLI), an effective therapy for Hodgkin's disease, has been used more recently to treat intractable rheumatoid arthritis. TLI is an immunosuppressive therapy, and after treatment, there is usually preferential repopulation of PBMC with suppressor CD8+ lymphocytes. We tested the effect of TLI on SIV infection of macaques as a method of specifically targeting cells expressing virus in lymph nodes. Two macaques with lymphadenopathy, that had been infected with SIV for 13 and 21 mos, received 3420 rad, first to the mantle and then to the inverted-Y field, in fractionated doses of 180 rad administered 5 days/wk. The animals were monitored daily for weight and clinical status; twice weekly for CBC, differential counts, and lymphocyte subsets; and every 2 wks for virologic and immunologic status. Despite severe depletion of all lymphocyte subsets, both animals remained clinically well, had no side effects, and weights were maintained at pre-TLI levels. During TLI lymphadenopathy regressed to undetectable levels, and there was no apparent increase in SIV viremia or p27 in blood, or any evidence of infection by other pathogens. After 3 months of follow-up, both animals have increased numbers of all lymphocyte subsets, with preferential repopulation of B cells and CD8+ T cells. One animal had a transient increase in the size of one axillary lymph node, while the second animal's lymphadenopathy is apparently relapsing. Semi-quantitative PCR analyses and functional studies of lymphocytes are in progress. The results to date indicate that TLI is well-tolerated in SIV-infected macaques, but long-term follow-up is required to assess potential therapeutic benefit and long-term effects on virus burden.
Keywords: Animal B-Lymphocytes/IMMUNOLOGY/RADIATION EFFECTS Human HIV Infections/IMMUNOLOGY/*RADIOTHERAPY Immunosuppression/METHODS Lymph Nodes/*RADIATION EFFECTS Lymphocyte Subsets/IMMUNOLOGY/RADIATION EFFECTS Macaca Radiotherapy/METHODS Radiotherapy Dosage Simian Acquired Immunodeficiency Syndrome/IMMUNOLOGY/ *RADIOTHERAPY SIV T-Lymphocytes/IMMUNOLOGY/RADIATION EFFECTS Time Factors ABSTRACT 940730
M9470882
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Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.
