Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.
Serosurvey of nonhuman primates in Kenya: evidence for SIVsmm-related infection in a baboon.
Symp Nonhum Primate Models AIDS. 1993 Sep 19-22;11:abstract no. 48. Unique Identifier : AIDSLINE PRIM11/94191643 Novembre FJ; Chege G; Ishahakia M; McClure HM; Yerkes Regional Primate Research Center, Emory University,; Atlanta, GA.
Abstract:
The Yerkes Regional Primate Research Center and the Institute of Primate Research have an ongoing collaboration to examine the prevalence of lentiviral infections in feral nonhuman primates. A recent serosurvey was conducted with six species of nonhuman primates utilizing a standard HIV-2 ELISA kit. Positive samples by ELISA were then confirmed by western blot utilizing SIVsmm9 virus or SIVagm virus as antigen. The results are as follows: Of 28 Blue Sykes, 11 (39%) tested positive, with 10 being confirmed by western blot. Of 7 Red tails, 1 (14%) tested positive and also confirmed. Of 13 DeBrazza monkeys, 0 (0%) tested positive. Of 29 Vervets, 9 (31%) tested positive, with 5 confirming by western blot. Of 11 Highland Sykes, 3 (27%) tested positive, with all 3 being confirmed. Of 106 Baboons, 2 (2%) tested positive and 1 (#1621) confirmed by western blot. These results indicate a moderate prevalence of natural lentiviral infection in feral primates. Baboon 1621 was further evaluated after importation to the Yerkes Center. After confirmation of the seropositive status of this baboon (also seropositive for STLV-I), attempts were made at SIV isolation. All attempts thus far have been negative. These virus isolations included depletion of CD8+ cells, and co-culturing with a number of cell types (Baboon PBMC, CEMss, Molt 4 Cl.8, U937, CEMx174, and Hut 78). Subsequently, a lymph node biopsy was obtained for: 1) virus isolation; 2) macaque inoculation; and 3) genomic DNA preparation. Virus isolation was negative. Macaques inoculated with lymph node cells (LNC) have not seroconverted to SIV (but have seroconverted to STLV-I). DNA obtained from PBMC and LNC were used in southern blot and PCR experiments to detect SIV-specific sequences. Positive results were obtained with southern blot and with PCR using conserved LTR primers, but not Gag primers. Sequence analysis of amplified products revealed similarities to the HIV-2/SIVsmm/SIVmac group of primate lentiviruses.
Keywords: Animal Animals, Wild Antibodies, Viral/*BLOOD Blotting, Western Cell Line DNA Primers Enzyme-Linked Immunosorbent Assay Gene Products, gag/GENETICS Human HIV-2 Kenya/EPIDEMIOLOGY Papio Polymerase Chain Reaction Repetitive Sequences, Nucleic Acid Simian Acquired Immunodeficiency Syndrome/DIAGNOSIS/*EPIDEMIOLOGY/ IMMUNOLOGY Support, U.S. Gov't, P.H.S. SIV/*ISOLATION & PURIF STLV/ISOLATION & PURIF T-Lymphocyte Subsets/IMMUNOLOGY ABSTRACT 940730
M9470875
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Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.
