Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.
The mucosal barrier selects for SIV with unique envelope sequences.
Symp Nonhum Primate Models AIDS. 1993 Sep 19-22;11:abstract no. 50. Unique Identifier : AIDSLINE PRIM11/94191646 Trivedi P; Emau P; Pauza CD; Wisconsin Regional Primate Research Center, University of; Wisconsin, Madison 53706.
Abstract:
One year old juvenile rhesus monkeys were infected intravenously or intrarectally with varying doses of SIVmac. This biological isolate proved to be a complex mixture of viruses based on extensive sequence analysis of viral long terminal repeats. Peripheral blood mononuclear cells (PBMC) were collected from infected animals at 8 or 16 weeks after inoculation; DNA was purified and a 1.2Kb portion of the SIVenv gene, including the V1-V5 regions, was amplified by polymerase chain reaction. The amplified env genes were cloned and sequenced with primers specific to the SIVmac239 sequence. In vitro infection of CEMX174, followed by amplification and cloning, yielded 10 clones of the env region. Between 10 and 20 clones were prepared from each infected animal (one i.v.-infected animal, one animal infected i.r. with 100 Infectious Doses, and one animal infected i.r. with 0.1ID). Sequences were determined for both strands of each clone and for uncloned PCR amplified products. The results were compared with the known env sequences of SIVmac251 and SIVmac239. We identified env sequences unique to intrarectally infected animals; these sequences were defined by a pattern of amino acid substitutions in both variable and constant regions. Sequences of env genes from ir-infected animals varied somewhat in comparison with each other yet, were distinct as a group from predominant sequences in i.v.-infected animals or in CEMX174 cells infected in vitro. These data suggest that unique env genes are associated with intramucosal transmission of SIVmac in rhesus monkeys; this property may be similar to the ways in which env sequence variation affects cellular tropism of virus. Our data promote the development of mucosal vaccines by highlighting a restricted group of viral envelope sequence associated with efficient intramucosal transmission in the rhesus monkey.
Keywords: Animal Cell Line Cloning, Molecular DNA Primers DNA, Viral/ISOLATION & PURIF *Genes, env Intestinal Mucosa/*MICROBIOLOGY Macaca mulatta Polymerase Chain Reaction/METHODS Rectum Simian Acquired Immunodeficiency Syndrome/MICROBIOLOGY/ *TRANSMISSION SIV/*GENETICS/*PATHOGENICITY Variation (Genetics) ABSTRACT 940730
M9470872
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Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.
