ADCC function in sera from infected but not from immunized rhesus monkeys.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

ADCC function in sera from infected but not from immunized rhesus monkeys.

Symp Nonhum Primate Models AIDS. 1993 Sep 19-22;11:abstract no. 61. Unique Identifier : AIDSLINE PRIM11/94191657
Ohkawa S; Wilson LA; Miller M; Montelaro R; Martin L; Murphey-Corb M; Tulane Regional Primate Research Center, Covington, LA 70433.

Abstract: Using vaccinia recombinants, we prepared target cells expressing SIV env specific for SIV/B670 or SIVmac251. Serum samples collected from SIV/B670 infected, SIVmacBK28 infected, or SIVmac251 recombinant glycoprotein immunized monkeys were assessed for complement-mediated lysis (ACC) or antibody dependent cellular cytolysis (ADCC). ACC against SIVmac251 env-expressing targets occurred in sera from SIV/B670 infected, SIVmacBK28 infected and glycoprotein immunized monkeys. ADCC was observed in sera from infected but not immunized monkeys. In a competitive ELISA inhibition assay, antibody reactivity with native gp110 was blocked partially by denatured gp140, indicating that a portion of antibodies recognized non-conformational epitopes but the majority of antibodies recognized conformational epitopes. ACC was blocked with denatured gp140 as well as native gp110, indicating that ACC mediating antibodies recognized mainly non-conformational epitopes. Competitive inhibition using three fragments of envelope glycoprotein indicated that epitope(s) recognized by ACC antibody lie between AA 214-471. Gp140 did not compete as well as gp110 in the ADCC assay, indicating that the majority of ADCC antibody recognized conformational epitopes. Inhibition with fragments indicated a portion of the ADCC epitopes lie between AA 52-214. The majority of antibody cross reactive between isolates recognized conformational epitopes because native but not denatured glycoprotein inhibited the activity. Chromatography of rhesus IgG resulted in three subclass enriched fractions. IgG-I mediated ACC and ADCC. IgG-II mediated ADCC but no ACC. Further examination of these activities in protected and unprotected vaccinates will be useful for identifying future responses which are protective.

Keywords: Animal Antibodies, Viral/BLOOD *Antibody-Dependent Cell Cytotoxicity Comparative Study Complement/IMMUNOLOGY Cytotoxicity, Immunologic Enzyme-Linked Immunosorbent Assay Gene Products, env/IMMUNOLOGY Macaca mulatta Simian Acquired Immunodeficiency Syndrome/*BLOOD/*IMMUNOLOGY/ PREVENTION & CONTROL Vaccines, Synthetic/*IMMUNOLOGY Viral Envelope Proteins/IMMUNOLOGY Viral Vaccines/*IMMUNOLOGY ABSTRACT
940730
M9470861

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