Scanning tunnelling microscopy observations of biomolecules on layered materials. NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

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Scanning tunnelling microscopy observations of biomolecules on layered materials.

Faraday Discuss. 1992;(94):183-97. Unique Identifier : AIDSLINE MED/94004063
Jungblut H; Campbell SA; Giersig M; Muller DJ; Lewerenz HJ; Hahn-Meitner-Institut Berlin, Bereich Photochemische; Energieumwandlung, Germany.

Abstract: Scanning tunnelling microscopy (STM) has been performed on the reverse transcriptases of the human immunodeficiency virus (HIV-1) and the moloney murine leukaemia virus (MuLV). The biological molecules are adsorbed on n-type semiconducting MoTe2. The p66 (66 kD) subunit of the RT of HIV-1 is imaged by STM. Both STM and processed transmission electron microscopy (TEM) data show a spherical and horseshoe-like shape of external diameter ca. 65 A, depending on the angle of observation. The STM results show a larger diameter which is related to the curvature radius of the tip of the probing needle. The RTs of HIV-1 and MuLV exhibit a circular hole of ca. 20 A diameter in accordance with structure predictions and functioning considerations. The surface-molecule interaction is discussed in terms of the electronic properties of the semiconductor surface including the influence of small defect sites at the layered crystal surface.
Keywords: Dialysis HIV-1/ENZYMOLOGY Microscopy, Scanning Tunneling Moloney Leukemia Virus/ENZYMOLOGY Neutrons RNA-Directed DNA Polymerase/*ANALYSIS Scattering, Radiation Spectrophotometry, Ultraviolet JOURNAL ARTICLEKWDdialysishiv-1/enzymologymicroscopy,scanningtunnelingmoloneyleukemiavirus/enzymologyneutronsrna-directeddnapolymerase/KWDanalysisscattering,radiationspectrophotometry,ultravioletjournalarticle
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M9410809

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