A simple method to distinguish between simian immunodeficiency virus isolates by restriction analysis of PCR products. NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

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A simple method to distinguish between simian immunodeficiency virus isolates by restriction analysis of PCR products.

DNA Cell Biol. 1993 Sep;12(7):617-22. Unique Identifier : AIDSLINE MED/94000387
Blackbourn DJ; Sutjipto S; Doi RH; Chuang RY; Department of Medical Pharmacology and Toxicology, School of; Medicine, University of California, Davis 95616.

Abstract: A simple method to distinguish between simian immunodeficiency virus (SIV) isolates of experimentally infected rhesus macaques is reported. Peripheral blood mononuclear cells (PBMC) were prepared from a rhesus macaque infected with SIVStM isolated originally from a stump-tailed macaque, or from a rhesus monkey infected with SIVSM from a sooty mangabey monkey. PBMC were cocultivated with CEM x 174 cells and a region of the SIV envelope (env) gene was amplified by the polymerase chain reaction (PCR) from cDNA of infected cocultivation cells. Restriction enzyme digestion analysis of the PCR products enabled SIVStM and SIVSM to be differentiated from each other, and from a molecular clone of SIVMAC, SIVMAC239, originally isolated from an infected rhesus macaque. Furthermore, when SIVSM and SIVStM were introduced into the same animal, restriction enzyme analysis of the PCR product amplified from cocultivation cells of this rhesus macaque suggested that the animal was superinfected.
Keywords: Animal Cell Line Cercocebus atys DNA Restriction Enzymes Macaca mulatta Macaca nemestrina Monocytes/MICROBIOLOGY Polymerase Chain Reaction Simian Acquired Immunodeficiency Syndrome/*MICROBIOLOGY Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. SIV/*ISOLATION & PURIF JOURNAL ARTICLEKWDanimalcelllinecercocebusatysdnarestrictionenzymesmacacamulattamacacanemestrinamonocytes/microbiologypolymerasechainreactionsimianacquiredimmunodeficiencysyndrome/KWDmicrobiologysupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDsiv/KWDisolation&purifjournalarticle
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M9410798

Copyright © 1994 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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