Constitutive interleukin-8 (IL-8) production by normal and malignant human B-cells, HTLV transformed T cells and other cell types (Meeting abstract). NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

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Constitutive interleukin-8 (IL-8) production by normal and malignant human B-cells, HTLV transformed T cells and other cell types (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 34:A2637 1993. Unique Identifier : AIDSLINE ICDB/94693579
Srivastava MD; Srivastava R; Srivastava BI; Dept. of Lab. Medicine, Roswell Park Cancer Inst., Buffalo, NY; 14263

Abstract: The culture supernatants from 36 human cell lines and from 4-5 day culture in RPMI 1640-5% fetal calf serum of purified leukemic cells from 16 patients (7 CLL, 2 PLL, 4 AML, 1 AMMOL, 2 dendritic cell leukemia) were examined for IL-8 using R and D ELISA Kits. IL-8 production was found for 14/15 B-cell lines (5 normal, 2 AML origin, 1 pre-B ALL, 2 CLL with trisomy 12, 2 HTLV-I+, 1 HTLV-II+, 1/2 BL), 4/11 T-cell lines (3/6 ATL, 1 HTLV-II+, 0/4 ALL), myeloid line HL 60, 3/3 ovarian carcinoma, 1/1 endometriosis (MD-E), 2/2 normal fibroblast (BG7, BG9), 0/2 C ALL lines (IL-8 range 100-6000 pg/ml) and for cells from all leukemia patients (IL-8 range 1000-7600 pg/ml). The cells from some of the above positive samples examined by indirect immunofluorescence using IL-8 antibodies gave positive reaction. cDNA probe for IL-8 detected 3.5-kb EcoR1 DNA fragment from all 11 cell types and 1.8-kb mRNA from 2 cell types examined. These results indicate IL-8 production by a greater variety of cells than believed previously, which indicates possibilities for new IL-8-mediated immune functions by such cells as B cells.
Keywords: B-Lymphocytes/*METABOLISM Cell Line Cell Transformation, Viral DNA Probes Fluorescent Antibody Technique Human *HTLV-I *HTLV-II Interleukin-8/*BIOSYNTHESIS/IMMUNOLOGY RNA, Messenger/METABOLISM T-Lymphocytes/*METABOLISM Tumor Cells, Cultured ABSTRACTKWDb-lymphocytes/KWDmetabolismcelllinecelltransformation,viraldnaprobesfluorescentantibodytechniquehumanKWDhtlv-iKWDhtlv-iiinterleukin-8/KWDbiosynthesis/immunologyrna,messenger/metabolismt-lymphocytes/KWDmetabolismtumorcells,culturedabstract
940228
M9420830

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